Epitopes described in "Immunization with a tetraepitopic lipid core peptide vaccine construct induces broadly protective immune responses against group A streptococcus."

Article Authors:Colleen Olive; Mei-Fong Ho; Joanne Dyer; Douglas Lincoln; Nadia Barozzi; Istvan Toth; Michael F Good
Article Title:Immunization with a tetraepitopic lipid core peptide vaccine construct induces broadly protective immune responses against group A streptococcus.
Reference Detail
Reference ID:1002168
Abstract:BACKGROUND: The development of a vaccine to prevent infection with group A streptococcus (GAS) is hampered by the widespread diversity of circulating GAS strains and M protein types, and it is widely believed that a multivalent vaccine would provide better protective immunity. METHODS: We investigated the efficacy of incorporating 3 M protein serotypic amino-terminal epitopes from GAS isolates that are common in Australian Aboriginal communities and a conformational epitope from the conserved carboxy-terminal C-repeat region into a single synthetic lipid core peptide (LCP) vaccine construct in inducing broadly protective immune responses against GAS after parenteral delivery to mice. RESULTS: Immunization with the tetraepitopic LCP vaccine construct led to high titers of systemic, antigen-specific IgG responses and the induction of broadly protective immune responses, as was demonstrated by the ability of immune serum to opsonize multiple GAS strains. Systemic challenge of mice with a lethal dose of GAS given 60 or 300 days after primary immunization showed that, compared with the control mice, the vaccinated mice were significantly protected against GAS infection, demonstrating that the vaccination stimulated long-lasting protective immunity. CONCLUSIONS: These data support the efficacy of the LCP vaccine delivery system in the development of a synthetic, multiepitopic vaccine for the prevention of GAS infection.
Affiliations:Cooperative Research Centre for Vaccine Technology, Queensland Institute of Medical Research, Brisbane, Australia. colleenO@qimr.edu.au
Reference Type:Literature
PubMed ID:16703510
Journal:J Infect Dis
Journal Volume:193
Article Pages:1666-76
Journal ISSN:1537-6613
Article Chemical List:Antibodies, Bacterial;Antigens, Bacterial;Bacterial Outer Membrane Proteins;Carrier Proteins;Epitopes;Immunoglobulin G;Lipids;Opsonin Proteins;Streptococcal Vaccines;Vaccines, Subunit;streptococcal M protein
Article MeSH List:Animals; Antibodies, Bacterial(blood); Antigens, Bacterial(immunology); Bacterial Outer Membrane Proteins(immunology); Carrier Proteins(immunology); Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Epitopes(immunology); Immunoglobulin G(blood); Lipids(chemistry; immunology); Mice; Microscopy, Fluorescence; Molecular Structure; Opsonin Proteins(blood); Streptococcal Infections(immunology; prevention & control); Streptococcal Vaccines(administration & dosage; immunology); Streptococcus pyogenes(immunology); Survival Analysis; Vaccines, Subunit(administration & dosage; chemistry; immunology)
Curation Last Updated:2014-10-03 20:16:48