Epitopes described in "Dextramers: new generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells."

Article Authors:Pascal Batard; Daniel A Peterson; Estelle Devêvre; Philippe Guillaume; Jean-Charles Cerottini; Donata Rimoldi; Daniel E Speiser; Lars Winther; Pedro Romero
Article Title:Dextramers: new generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells.
Reference Detail
Reference ID:1004534
Abstract:Direct identification as well as isolation of antigen-specific T cells became possible since the development of "tetramers" based on avidin-fluorochrome conjugates associated with mono-biotinylated class I MHC-peptide monomeric complexes. In principle, a series of distinct class I MHC-peptide tetramers, each labelled with a different fluorochrome, would allow to simultaneously enumerate as many unique antigen-specific CD8(+) T cells. Practically, however, only phycoerythrin and allophycocyanin conjugated tetramers have been generally available, imposing serious constraints for multiple labeling. To overcome this limitation, we have developed dextramers which are multimers based on a dextran backbone bearing multiple fluorescein and streptavidin moieties. Here we demonstrate the functionality and optimization of these new probes on human CD8(+) T cell clones with four independent antigen specificities. Their applications to the analysis of relatively low frequency antigen-specific T cells in peripheral blood, as well as their use in fluorescence microscopy, are demonstrated. The data show that dextramers produce a stronger signal than their fluoresceinated tetramer counterparts. Thus, these could become the reagents of choice as the antigen-specific T cell labeling transitions from basic research to clinical application.
Reference Type:Literature
PubMed ID:16516226
Journal:J Immunol Methods
Journal Volume:310
Article Pages:136-48
Journal ISSN:0022-1759
Article Chemical List:Antigens, Neoplasm;Epitopes, T-Lymphocyte;Fluorescent Dyes;HLA-A2 Antigen;MART-1 Antigen;MLANA protein, human;Neoplasm Proteins;Phosphoproteins;SM protein, Human herpesvirus 4;Trans-Activators;Viral Matrix Proteins;Viral Proteins;Dextrans
Article MeSH List:Antigens, Neoplasm; CD8-Positive T-Lymphocytes(immunology); Dextrans(chemistry; immunology); Epitopes, T-Lymphocyte(immunology); Flow Cytometry; Fluorescent Dyes(chemistry); HLA-A2 Antigen(chemistry; immunology); Humans; MART-1 Antigen; Microscopy, Fluorescence; Molecular Weight; Neoplasm Proteins(chemistry; immunology); Phosphoproteins(chemistry; immunology); T-Lymphocytes, Cytotoxic(immunology); Trans-Activators(chemistry; immunology); Viral Matrix Proteins(chemistry; immunology); Viral Proteins(chemistry; immunology)
Curation Last Updated:2015-04-22 20:01:20