Epitopes described in "Specific IgE determination to epitope peptides of omega-5 gliadin and high molecular weight glutenin subunit is a useful tool for diagnosis of wheat-dependent exercise-induced anaphylaxis."

Reference
Article Authors:Hiroaki Matsuo; Kunie Kohno; Hiroyuki Niihara; Eishin Morita
Article Title:Specific IgE determination to epitope peptides of omega-5 gliadin and high molecular weight glutenin subunit is a useful tool for diagnosis of wheat-dependent exercise-induced anaphylaxis.
Reference Detail
Reference ID:1016094
Abstract:Wheat omega-5 gliadin and a high m.w. glutenin subunit (HMW-glutenin) have been reported as major allergens in wheat-dependent exercise-induced anaphylaxis. A simultaneous detection of specific IgE to epitope sequences of both proteins is considered to be a reliable method for diagnosis of wheat-dependent exercise-induced anaphylaxis. However, the IgE-binding epitope of HMW-glutenin remains unknown. The aim of this study was to determine the IgE-binding epitopes of HMW-glutenin to establish a useful system of identifying patients with wheat-dependent exercise-induced anaphylaxis. For determination of IgE-binding epitopes of HMW-glutenin overlapping peptides were synthesized and reactivities of IgE Abs in the sera of patients to those peptides were analyzed. Three IgE-binding epitopes, QQPGQ, QQPGQGQQ, and QQSGQGQ, were identified within primary sequence of HMW-glutenin. Epitope peptides, which include IgE-binding sequences of omega-5 gliadin and a HMW-glutenin, were synthesized and peptide-specific IgE Abs were measured by CAP-System fluorescent enzyme immunoassay. Twenty-nine of 30 patients with wheat-dependent exercise-induced anaphylaxis had specific IgE Abs to these epitope peptides. None of the 25 sera from healthy subjects reacted to both epitope peptides. Twenty-five patients with atopic dermatitis who had specific IgE to wheat and/or gluten had very low or nonexistent levels of epitope peptide-specific IgE Abs. These results indicated that measurement of IgE levels specific to epitope peptides of omega-5 gliadin and HMW-glutenin is useful as an in vitro diagnostic method for the assessment of patients with wheat-dependent exercise-induced anaphylaxis.
Affiliations:Department of Dermatology, Shimane University School of Medicine, Izumo, Japan. hmatsuo@med.shimane-u.ac.jp
Date:2005
Reference Type:Literature
PubMed ID:16339549
Journal:J Immunol
Journal Volume:175
Article Pages:8116-22
Journal ISSN:0022-1767
Article Chemical List:Allergens;Antibodies;Antigens, Plant;Epitopes;Protein Subunits;omega-5 gliadin, wheat;Immunoglobulin E;Glutens;Gliadin;glutenin
Article MeSH List:Allergens(immunology); Amino Acid Sequence; Anaphylaxis(diagnosis; etiology); Antibodies(analysis); Antigens, Plant; Case-Control Studies; Dermatitis, Atopic(diagnosis); Epitope Mapping; Epitopes(diagnostic use); Exercise; Gliadin(immunology); Glutens(immunology); Humans; Immunoenzyme Techniques(standards); Immunoglobulin E(analysis; immunology); Protein Subunits(immunology); Wheat Hypersensitivity(complications; diagnosis)
Curation Last Updated:2014-07-16 22:14:09