Epitopes described in "Abeta42 immunization in Alzheimer's disease generates Abeta N-terminal antibodies."

Article Authors:Michael Lee; Frederique Bard; Kelly Johnson-Wood; Celeste Lee; Kang Hu; Sue G Griffith; Ronald S Black; Dale Schenk; Peter Seubert
Article Title:Abeta42 immunization in Alzheimer's disease generates Abeta N-terminal antibodies.
Reference Detail
Reference ID:1008554
Abstract:Serum samples from Alzheimer's disease (AD) patients immunized with Abeta42 (AN1792) were analyzed to determine the induced antibody properties including precise amyloid-beta peptide (Abeta) epitopes and amyloid plaque-binding characteristics. The predominant response in these patients is independent of whether or not meningoencephalitis developed and is against the free amino terminus of Abeta. The immunostaining of amyloid plaques in brain tissue by patient sera is adsorbable by a linear Abeta1-8 peptide, demonstrating that the antibodies are directed predominantly to this epitope and not dependent on Abeta conformations or aggregates specific to plaques. Furthermore, the antibodies are not capable of binding amyloid precursor protein and would be predicted to be competent in facilitating clearance of amyloid plaques in AD brains.
Affiliations:Elan Pharmaceuticals, Inc., So. San Francisco, CA 94080, USA.
Reference Type:Literature
PubMed ID:16130106
Journal:Ann Neurol
Journal Volume:58
Article Pages:430-5
Journal ISSN:1531-8249
Article Chemical List:AN-1792;Amyloid beta-Peptides;Epitopes;Peptide Fragments;amyloid beta-protein (1-42)
Article MeSH List:Alzheimer Disease(immunology; prevention & control); Amyloid beta-Peptides(administration & dosage; immunology; metabolism); Antibody Specificity; Blotting, Western(methods); Dose-Response Relationship, Immunologic; Epitope Mapping; Epitopes(immunology); Humans; Immunization(adverse effects; methods); Immunohistochemistry(methods); Meningoencephalitis(blood; etiology; immunology); Peptide Fragments(immunology; metabolism)
Curation Last Updated:2014-10-03 21:02:17