Epitopes described in "Antibody responses against galactocerebroside are potential stage-specific biomarkers in multiple sclerosis."

Article Authors:Til Menge; Patrice H Lalive; Hans-Christian von B├╝dingen; Bruce Cree; Stephen L Hauser; Claude P Genain
Article Title:Antibody responses against galactocerebroside are potential stage-specific biomarkers in multiple sclerosis.
Reference Detail
Reference ID:1015584
Abstract:BACKGROUND: Galactocerebroside, the major glycolipid of central nervous system myelin, is a known target for pathogenic demyelinating antibody responses in experimental allergic encephalomyelitis (EAE), the animal model of multiple sclerosis (MS). OBJECTIVE: To address the importance of anti-galactocerebroside (alpha-GalC) antibodies in MS and to evaluate them as biomarkers of disease. METHODS: alpha-GalC IgGs were quantified from sera of patients with MS and in marmoset EAE by a new immunosorbent assay. RESULTS: We report a significant difference in serum alpha-GalC IgG titers between patients with relapsing-remitting (RR)-MS and healthy controls (HCs; P < .001). The frequencies of alpha-GalC antibody-positive subjects (alpha-GalC titers > or = mean HC titers+3 SD) are also significantly elevated in RR-MS compared with HC (40% vs 0%; P = .0033). Immunoaffinity purified alpha-GalC IgGs from human serum bind to cultured human oligodendrocytes, indicating that the ELISA detects a biologically relevant epitope. Corroborating these findings, alpha-GalC antibody responses in marmoset EAE were similarly found to be specifically associated with the RR forms and not the peracute or progressive forms, in contrast with other anti-myelin antibodies (P = .0256). CONCLUSION: (1) alpha-GalC antibodies appear MS-specific and are not found in healthy subjects, unlike antibodies against myelin proteins; (2) when present, alpha-GalC antibodies identify mostly RR-MS and may be an indicator of ongoing disease activity. This novel assay is a suitable and valuable method to increase accuracy of diagnosis and disease staging in MS.
Affiliations:Department of Neurology, University of California San Francisco, San Francisco, CA 94143-0114, USA.
Reference Type:Literature
PubMed ID:16083805
Journal:J Allergy Clin Immunol
Journal Volume:116
Article Pages:453-9
Journal ISSN:0091-6749
Article Chemical List:Biological Markers;Galactosylceramides;Immunoglobulin G;galactocerebroside
Article MeSH List:Adult; Aged; Animals; Biological Markers; Callithrix; Encephalomyelitis, Autoimmune, Experimental(immunology); Enzyme-Linked Immunosorbent Assay; Female; Galactosylceramides(immunology); Humans; Immunoglobulin G(blood); Immunohistochemistry; Male; Middle Aged; Multiple Sclerosis(diagnosis; immunology)
Curation Last Updated:2016-04-11 21:01:31