Epitopes described in "Use of Peptide library screening to detect a previously unknown linear diagnostic epitope: proof of principle by use of lyme disease sera."

Article Authors:Carl V Hamby; Marta Llibre; Sandeepa Utpat; Gary P Wormser
Article Title:Use of Peptide library screening to detect a previously unknown linear diagnostic epitope: proof of principle by use of lyme disease sera.
Reference Detail
Reference ID:1001701
Abstract:Diagnostic peptides previously isolated from phage-displayed libraries by affinity selection with serum antibodies from patients with Lyme disease were found to give reproducible serum reactivity patterns when tested in two different enzyme-linked immunosorbent assay formats. In addition, the hypothetical possibility that peptides selected by this type of "epitope discovery" technique might identify the original antigens eliciting antibody responses was tested by searching for sequence similarities in bacterial protein databases. In support of this hypothesis, our search uncovered similarities between peptides representing two different sequence motifs and sequences in the VlsE and BBA61 antigens of Borrelia burgdorferi. Utilizing synthetic peptides, we verified that the sequence KAASKETPPALNK, located at the C terminus of the VlsE antigen, had the same reactivity pattern to sera from patients with extracutaneous Lyme disease as the diagnostic peptide SKEKPPSLNWPA, with which it shared a 7-amino-acid-residue match (consensus residues are underlined). A peptide with conservative mutations of five of the consensus residues was nonreactive, strongly suggesting that the VlsE sequence represents the epitope that originally elicited antibody responses in these patients. The diagnostic sensitivity of this new VlsE epitope was relatively low (30%) compared to that (100%) of the well-documented C6 diagnostic peptide of VlsE when tested in our small cohort of 10 patients with Lyme disease. Nonetheless, the identification of this previously unknown epitope serves as a proof of the principle of the hypothetical ability of "epitope discovery" techniques to detect specific microbial antigens with diagnostic relevance in infectious diseases.
Affiliations:Department of Microbiology and Immunology, New York Medical College, Valhalla, NY 10595, USA. hamby@nymc.edu.
Reference Type:Literature
PubMed ID:16002626
Journal:Clin Diagn Lab Immunol
Journal Volume:12
Article Pages:801-7
Journal ISSN:1098-6588
Article Chemical List:gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@b72a210;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@12edce4f;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@3a83ae3e;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@1b248675;gov.nih.nlm.ncbi.www.jaxb.impl.NameOfSubstanceImpl@7af7793e
Article MeSH List:Amino Acid Sequence; Antibodies, Bacterial(immunology); Antigens, Bacterial(genetics; immunology); Borrelia burgdorferi(genetics; immunology); Epitopes(diagnostic use; genetics; immunology); Humans; Lyme Disease(diagnosis; immunology); Peptide Library; Peptides(diagnostic use; genetics; immunology); Sensitivity and Specificity
Curation Last Updated:2015-01-17 21:10:50