Epitopes described in "T-cell recognition and cytokine profile induced by melanocyte epitopes in patients with HLA-DRB1*0405-positive and -negative Vogt-Koyanagi-Harada uveitis."

Reference
Article Authors:Francisco Max Damico; Edecio Cunha-Neto; Anna Carla Goldberg; Leo Kei Iwai; Maria Lucia Marin; Juergen Hammer; Jorge Kalil; Joyce Hisae Yamamoto
Article Title:T-cell recognition and cytokine profile induced by melanocyte epitopes in patients with HLA-DRB1*0405-positive and -negative Vogt-Koyanagi-Harada uveitis.
Reference Detail
Reference ID:1017163
Abstract:PURPOSE: Vogt-Koyanagi-Harada disease (VKH), an autoimmune disease targeted against melanocytes, is associated with HLA-DRB1*0405. This study was undertaken to analyze T-cell recognition and the cytokine expression profile induced by melanocyte epitopes in HLA-DRB1*0405-positive and -negative patients with VKH uveitis. METHODS: Peripheral blood mononuclear cells (PBMC) proliferation and Th1 (IFN-gamma) and Th2 (IL-4 and IL-5) cytokine production were analyzed in HLA-DRB1*0405-positive (n = 12) and -negative (n = 22) patients with VKH and HLA-DRB1*0405-positive (n = 9) and -negative (n = 8) control subjects in response to human melanoma cell line lysate (HMCLL) and 28 synthetic peptides derived from the human melanocyte differentiation proteins TYR, TRP1, TRP2, and Pmel17. The peptides were selected using the TEPITOPE algorithm, based on their predicted binding to HLA-DRB1*0405 and to the non-disease-related HLA-DRB1*15. RESULTS: HMCLL was recognized exclusively by the patients' PBMC (44%) but not by those of the control subjects (P < 0.01). PBMC from patients with VKH recognized an increased breadth of melanocyte-derived peptides at lower peptide concentrations than in the control subjects (68% vs. 25%; P < 0.01, at 1 microM) and did not produce the Th2 cytokine IL-4 in response to disease-specific peptides (0% vs. 50%, P < 0.001). Five peptides were exclusively recognized in patients bearing HLA-DRB1*0405. Furthermore, HLA-DRB1*0405-bearing patients, but not those with HLA-DRB1*15, recognized an increased breadth of melanocyte epitopes in comparison to HLA-matched control subjects (60% vs. 28%; P < 0.05). CONCLUSIONS: These data indicate that patients with VKH are sensitized to melanocyte epitopes and display a peptide-specific Th1 cytokine response. In addition, the data indicate that patients bearing HLA-DRB1*0405 recognize a broader melanocyte-derived peptide repertoire, reinforcing the importance of this allele in susceptibility to the development of VKH disease.
Affiliations:Department of Ophthalmology, University of São Paulo School of Medicine, São Paulo, Brazil.
Date:2005
Reference Type:Literature
PubMed ID:15980237
Journal:Invest Ophthalmol Vis Sci
Journal Volume:46
Article Pages:2465-71
Journal ISSN:0146-0404
Article Chemical List:Epitopes;HLA-DR Antigens;HLA-DRB1 Chains;HLA-DRB1*04:05 antigen;Interleukin-5;Neoplasm Proteins;Peptides;Interleukin-4;Interferon-gamma
Article MeSH List:Adolescent; Adult; Child; Epitopes(immunology); Female; HLA-DR Antigens(immunology); HLA-DRB1 Chains; Humans; Interferon-gamma(metabolism); Interleukin-4(metabolism); Interleukin-5(metabolism); Lymphocyte Activation; Male; Melanocytes(immunology); Melanoma(immunology); Middle Aged; Neoplasm Proteins(immunology); Peptides(immunology); Th1 Cells(immunology); Th2 Cells(immunology); Tumor Cells, Cultured; Uveomeningoencephalitic Syndrome(immunology)
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