Epitopes described in "Borreliacidal OspC antibodies specific for a highly conserved epitope are immunodominant in human lyme disease and do not occur in mice or hamsters."

Article Authors:Steven D Lovrich; Dean A Jobe; Ronald F Schell; Steven M Callister
Article Title:Borreliacidal OspC antibodies specific for a highly conserved epitope are immunodominant in human lyme disease and do not occur in mice or hamsters.
Reference Detail
Reference ID:1001719
Abstract:Humans produce highly specific borreliacidal antibodies against outer surface protein C (OspC) shortly after infection with Borrelia burgdorferi sensu stricto. We previously demonstrated the epitope recognized by immunoglobulin M (IgM) and IgG OspC borreliacidal antibodies was located within the 50 amino acids nearest the carboxy (C) terminus. In this study, we show the immunodominant epitope is located in the highly conserved region within the seven C-terminal amino acids. Six early Lyme disease sera that contained borreliacidal activity and IgM and/or IgG OspC antibodies were chosen randomly and adsorbed with truncated OspC containing the 16 or 7 amino acids nearest the C terminus. Adsorptions with each truncated protein abrogated the borreliacidal activity completely. In addition, only small concentrations of OspC antibodies remained detectable by enzyme-linked immunosorbent assay and Western blotting. Moreover, borreliacidal OspC antibodies were not induced in laboratory mice or hamsters despite heavy infections with B. burgdorferi spirochetes. These findings confirm that borreliacidal antibodies comprise the majority of the IgM and IgG OspC antibody response in human Lyme disease and that the epitope is located in the highly conserved C terminus. In addition, rodent animal models appear to be inappropriate subjects for assessing the effectiveness of the epitope for serodiagnosis or as a human Lyme disease vaccine.
Affiliations:Microbiology Research Laboratory, Gundersen Lutheran Medical Center, 1300 Badger Street, La Crosse, WI 54601, USA.
Reference Type:Literature
PubMed ID:15939749
Journal:Clin Diagn Lab Immunol
Journal Volume:12
Article Pages:746-51
Journal ISSN:1098-6588
Article Chemical List:Antibodies, Bacterial;Antigens, Surface;Bacterial Outer Membrane Proteins;Bacterial Vaccines;Epitopes;Immunoglobulin G;Immunoglobulin M;Lipoproteins;OspA protein;Peptide Fragments;Recombinant Proteins
Article MeSH List:Adsorption; Amino Acid Sequence; Animals; Antibodies, Bacterial(blood); Antigens, Surface(chemistry; genetics; immunology); Bacterial Outer Membrane Proteins(chemistry; genetics; immunology); Bacterial Vaccines; Borrelia burgdorferi(immunology; pathogenicity); Conserved Sequence; Cricetinae; Epitopes(immunology); Female; Humans; Immunoglobulin G(blood); Immunoglobulin M(blood); Lipoproteins(chemistry; genetics; immunology); Lyme Disease(blood; immunology); Mice; Mice, Inbred C3H; Peptide Fragments(chemistry; genetics; immunology); Recombinant Proteins(chemistry; genetics; immunology); Species Specificity
Article Comments:ErratumIn(Clin Diagn Lab Immunol. 2005 Sep;12(9):1139 )
Curation Last Updated:2015-12-28 20:01:34