Epitopes described in "Expanded T cells from pancreatic lymph nodes of type 1 diabetic subjects recognize an insulin epitope."

Article Authors:Sally C Kent; Yahua Chen; Lisa Bregoli; Sue M Clemmings; Norma Sue Kenyon; Camillo Ricordi; Bernhard J Hering; David A Hafler
Article Title:Expanded T cells from pancreatic lymph nodes of type 1 diabetic subjects recognize an insulin epitope.
Reference Detail
Reference ID:1007476
Abstract:In autoimmune type 1 diabetes, pathogenic T lymphocytes are associated with the specific destruction of insulin-producing beta-islet cells. Identification of the autoantigens involved in triggering this process is a central question. Here we examined T cells from pancreatic draining lymph nodes, the site of islet-cell-specific self-antigen presentation. We cloned single T cells in a non-biased manner from pancreatic draining lymph nodes of subjects with type 1 diabetes and from non-diabetic controls. A high degree of T-cell clonal expansion was observed in pancreatic lymph nodes from long-term diabetic patients but not from control subjects. The oligoclonally expanded T cells from diabetic subjects with DR4, a susceptibility allele for type 1 diabetes, recognized the insulin A 1-15 epitope restricted by DR4. These results identify insulin-reactive, clonally expanded T cells from the site of autoinflammatory drainage in long-term type 1 diabetics, indicating that insulin may indeed be the target antigen causing autoimmune diabetes.
Affiliations:Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
Reference Type:Literature
PubMed ID:15889096
Journal Volume:435
Article Pages:224-8
Journal ISSN:1476-4687
Article Chemical List:Epitopes, T-Lymphocyte;HLA-DR Antigens;HLA-DRB1 Chains;Insulin;Receptors, Antigen, T-Cell
Article MeSH List:Alleles; Amino Acid Sequence; Case-Control Studies; Clone Cells(cytology; immunology); Diabetes Mellitus, Type 1(immunology; pathology); Epitopes, T-Lymphocyte(immunology); HLA-DR Antigens(immunology); HLA-DRB1 Chains; Humans; Insulin(immunology); Lymph Nodes(cytology; immunology); Molecular Sequence Data; Pancreas(immunology); Receptors, Antigen, T-Cell(chemistry; genetics; immunology); Substrate Specificity; T-Lymphocytes(cytology; immunology)
Curation Last Updated:2014-10-03 20:56:55