Epitopes described in "Water molecules in the antibody-antigen interface of the structure of the Fab HyHEL-5-lysozyme complex at 1.7 A resolution: comparison with results from isothermal titration calorimetry."

Reference
Article Authors:Gerson H Cohen; Enid W Silverton; Eduardo A Padlan; Fred Dyda; Jamie A Wibbenmeyer; Richard C Willson; David R Davies
Article Title:Water molecules in the antibody-antigen interface of the structure of the Fab HyHEL-5-lysozyme complex at 1.7 A resolution: comparison with results from isothermal titration calorimetry.
Reference Detail
Reference ID:1019500
Abstract:The structure of the complex between hen egg-white lysozyme and the Fab HyHEL-5 at 2.7 A resolution has previously been reported [Cohen et al. (1996), Acta Cryst. D52, 315-326]. With the availability of recombinant Fab, the X-ray structure of the complex has been re-evaluated at 1.7 A resolution. The refined structure has yielded a detailed picture of the Fab-lysozyme interface, showing the high complementarity of the protein surfaces as well as several water molecules within the interface that complete the good fit. The model of the full complex has improved significantly, yielding an R(work) of 19.5%. With this model, the structural results can be compared with the results of isothermal titration calorimetry. An attempt has been made to estimate the changes in bound waters that accompany complex formation and the difficulties inherent in using the crystal structures to provide the information necessary to make this calculation are discussed.
Date:2005
Reference Type:Literature
PubMed ID:15858274
Journal:Acta Crystallogr D Biol Crystallogr
Journal Volume:61
Article Pages:628-33
Journal ISSN:0907-4449
Article Chemical List:Antigen-Antibody Complex;Epitopes;Water;Muramidase
Article MeSH List:Antigen-Antibody Complex(chemistry); Calorimetry; Cloning, Molecular; Crystallography, X-Ray; Epitopes; Models, Molecular; Muramidase(chemistry); Protein Conformation; Quality Control; Water(chemistry)
Curation Last Updated:2014-07-16 22:29:31