Epitopes described in "Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens."

Article Authors:Robert Burns; Irina Luzina; Adnan Nasir; Constantine G Haidaris; Richard K Barth; Anthony A Gaspari
Article Title:Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens.
Reference Detail
Reference ID:1012693
Abstract:Background B7-1 transgenic mice exhibit exaggerated and persistent contact hypersensitivity responses compared with normal mice. Objective Because B7-1 and B7-2 deliver different costimulatory signals to T cells during antigen presentation, the purpose of this study was to compare B7-1 and B7-2 on keratinocytes and to compare their effects on contact hypersensitivity. Methods Contact hypersensitivity was studied in transgenic mice whose keratinocytes constitutively expressed B7-1, B7-2, or no costimulatory molecules (nontransgenic mice). Results B7-1 transgenic mice, and to a lesser extent B7-2 transgenic mice, developed exaggerated ear swelling responses after sensitization and challenge with haptens such as trinitrochlorobenzene or dinitrofluorobenzene. Ear swelling responses in B7-1 transgenic mice were characterized by the presence of markedly elevated inflammatory cytokine transcripts (IL-6, TNF-alpha, and lymphotoxin beta) as well as IL-10 compared with either B7-2 or nontransgenic mice. Hapten-specific IgE was detected by ELISA in B7-1 transgenic mice but not B7-2 transgenic or nontransgenic mice. Only B7-1 transgenic mice exhibited significant immediate type ear swelling responses to the hapten trinitrochlorobenzene. In addition, their sera can passively transfer cutaneous anaphylaxis to naive C57BL/6 mice, indicating that the hapten-specific IgE was relevant to the immediate ear swelling responses. Conclusion These data suggest that keratinocyte-derived costimulation mediated by B7-1 but not B7-2 results in the emergence of T H 2-lymphocyte immune responses to T H 1 haptens. Because human keratinocytes have been noted to express B7-1-like molecules in certain inflammatory skin diseases, this model may be important in understanding the pathophysiology of T H 2-lymphocyte-mediated skin diseases such as atopic dermatitis.
Reference Type:Literature
PubMed ID:15696100
Journal:J Allergy Clin Immunol
Journal Volume:115
Article Pages:383-90
Journal ISSN:0091-6749
Article Chemical List:Antigens, CD;Antigens, CD80;Antigens, CD86;Cd86 protein, mouse;Chlorobenzenes;Haptens;Membrane Glycoproteins;trichlorobenzene;Immunoglobulin E;Dinitrofluorobenzene
Article MeSH List:Animals; Antibody Formation; Antigens, CD(immunology); Antigens, CD80(immunology); Antigens, CD86; Chlorobenzenes(immunology); Dermatitis, Contact(immunology); Dinitrofluorobenzene(immunology); Ear Diseases(immunology); Edema(immunology); Haptens(immunology); Immunoglobulin E(biosynthesis); Keratinocytes(immunology); Membrane Glycoproteins(immunology); Mice; Mice, Inbred C57BL; Mice, Transgenic; Th1 Cells(immunology; metabolism)
Curation Last Updated:2016-05-11 20:05:07