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| Article Authors: | Robert Burns; Irina Luzina; Adnan Nasir; Constantine G Haidaris; Richard K Barth; Anthony A Gaspari |
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| Article Title: | Keratinocyte-derived, CD80-mediated costimulation is associated with hapten-specific IgE production during contact hypersensitivity to TH1 haptens. |
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| Reference ID: | 1012693 |
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| Abstract: | Background B7-1 transgenic mice exhibit exaggerated and persistent contact hypersensitivity responses compared with normal mice. Objective Because B7-1 and B7-2 deliver different costimulatory signals to T cells during antigen presentation, the purpose of this study was to compare B7-1 and B7-2 on keratinocytes and to compare their effects on contact hypersensitivity. Methods Contact hypersensitivity was studied in transgenic mice whose keratinocytes constitutively expressed B7-1, B7-2, or no costimulatory molecules (nontransgenic mice). Results B7-1 transgenic mice, and to a lesser extent B7-2 transgenic mice, developed exaggerated ear swelling responses after sensitization and challenge with haptens such as trinitrochlorobenzene or dinitrofluorobenzene. Ear swelling responses in B7-1 transgenic mice were characterized by the presence of markedly elevated inflammatory cytokine transcripts (IL-6, TNF-alpha, and lymphotoxin beta) as well as IL-10 compared with either B7-2 or nontransgenic mice. Hapten-specific IgE was detected by ELISA in B7-1 transgenic mice but not B7-2 transgenic or nontransgenic mice. Only B7-1 transgenic mice exhibited significant immediate type ear swelling responses to the hapten trinitrochlorobenzene. In addition, their sera can passively transfer cutaneous anaphylaxis to naive C57BL/6 mice, indicating that the hapten-specific IgE was relevant to the immediate ear swelling responses. Conclusion These data suggest that keratinocyte-derived costimulation mediated by B7-1 but not B7-2 results in the emergence of T H 2-lymphocyte immune responses to T H 1 haptens. Because human keratinocytes have been noted to express B7-1-like molecules in certain inflammatory skin diseases, this model may be important in understanding the pathophysiology of T H 2-lymphocyte-mediated skin diseases such as atopic dermatitis. |
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| Affiliations: | Department of Dermatology, University of Rochester School of Medicine, Rochester, USA. |
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| Date: | 2005 |
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| Reference Type: | Literature |
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| PubMed ID: | 15696100 |
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| Journal: | J Allergy Clin Immunol |
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| Journal Volume: | 115 |
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| Article Pages: | 383-90 |
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| Journal ISSN: | 0091-6749 |
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| Article Chemical List: | Antigens, CD;Antigens, CD80;Antigens, CD86;Cd86 protein, mouse;Chlorobenzenes;Haptens;Membrane Glycoproteins;trichlorobenzene;Immunoglobulin E;Dinitrofluorobenzene |
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| Article MeSH List: | Animals; Antibody Formation; Antigens, CD(immunology ); Antigens, CD80(immunology ); Antigens, CD86; Chlorobenzenes(immunology ); Dermatitis, Contact(immunology ); Dinitrofluorobenzene(immunology ); Ear Diseases(immunology ); Edema(immunology ); Haptens(immunology ); Immunoglobulin E(biosynthesis ); Keratinocytes(immunology ); Membrane Glycoproteins(immunology ); Mice; Mice, Inbred C57BL; Mice, Transgenic; Th1 Cells(immunology; metabolism ) |
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| Curation Last Updated: | 2013-05-28 21:28:07 |
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