Epitopes described in "Antibodies against OspA epitopes of Borrelia burgdorferi cross-react with neural tissue."

Article Authors:Armin Alaedini; Norman Latov
Article Title:Antibodies against OspA epitopes of Borrelia burgdorferi cross-react with neural tissue.
Reference Detail
Reference ID:290
Abstract:Neurological sequela of chronic Lyme disease include encephalopathy, myelopathy and peripheral neuropathy. These have generally been attributed to either persistent infection or pathogen-induced autoimmunity. In this study, we investigated the presence of cross-reactive human neural epitopes that share amino acid sequences with Borrelia burgdorferi OspA protein. Sequence similarity analysis was carried out by searching known cDNA sequences from brain tissue. The cDNA database search yielded three sequences that were identical to sequences in OspA. Corresponding peptides were synthesized and antibodies were generated against them in rabbits. Antibodies against two of the homologous OspA peptides were found to react with neurons in human brain, spinal cord and dorsal root ganglia by immunohistochemistry.
Affiliations:Department of Neurology and Neuroscience, Cornell University, 1300 York Ave., New York, NY 10021, USA. ara2004@med.cornell.edu
Reference Type:Literature
PubMed ID:15652419
Journal:J Neuroimmunol
Journal Volume:159
Article Pages:192-5
Journal ISSN:0165-5728
Article Chemical List:Antibodies, Bacterial;Antigens, Surface;Bacterial Outer Membrane Proteins;Bacterial Vaccines;Epitopes;Immune Sera;Lipoproteins;OspA protein
Article MeSH List:Animals; Antibodies, Bacterial(biosynthesis; metabolism); Antigens, Surface(immunology; metabolism); Bacterial Outer Membrane Proteins(immunology; metabolism); Bacterial Vaccines; Binding Sites, Antibody; Borrelia burgdorferi(immunology); Brain Chemistry(immunology); Cross Reactions; Epitopes(immunology; metabolism); Expressed Sequence Tags; Ganglia, Spinal(chemistry; immunology); Humans; Immune Sera(metabolism); Immunohistochemistry; Lipoproteins(immunology; metabolism); Neurons(chemistry; immunology); Rabbits; Sequence Homology, Nucleic Acid; Spinal Cord(chemistry; immunology)
Curation Last Updated:2015-12-28 20:01:21