Epitopes described in "Pooled peptides from HER-2/neu-overexpressing primary ovarian tumours induce CTL with potent antitumour responses in vitro and in vivo."

Reference
Article Authors:A D Gritzapis; S A Perez; C N Baxevanis; M Papamichail
Article Title:Pooled peptides from HER-2/neu-overexpressing primary ovarian tumours induce CTL with potent antitumour responses in vitro and in vivo.
Reference Detail
Reference ID:431
Abstract:Unfractionated peptides (MW: up to 10 kDa), derived from HLA-A2.1 positive (+) HER-2/neu-overexpressing primary tumour cell acid cell extracts (ACE), were successfully used to generate in vitro cytotoxic T lymphocytes (CTL). Primary tumour cells were collected from peritoneal malignant effusions of patients with ovarian cancer. Acid cell extracts-induced CTL specifically lysed in an HLA-A2-restricted manner HER-2/neu+ autologous primary tumour cells as well as HER-2/neu+ tumour cell lines. In addition, adoptive transfer of such CTL significantly prolonged the survival of SCID mice xenografted with HLA-A2.1+, HER-2/neu+ human breast and ovarian tumour cell lines. Acid cell extracts collected from HLA-A2.1+ HER-2/neu negative (-) primary ovarian tumours induced HLA-A2.1-restricted CTL with weak in vitro and in vivo antitumour capacity, suggesting that HER-2/neu peptides within ACE from HER-2/neu-overexpressing primary ovarian tumour cells are immunodominant. The results presented herein serve as a rationale for the initiation of vaccination studies in patients with HER-2/neu-overexpressing ovarian tumours utilising autologous tumour-derived ACE.
Affiliations:Cancer Immunology and Immunotherapy Center, Saint Savas Cancer Hospital, Athens, Greece.
Date:2005
Reference Type:Literature
PubMed ID:15583693
Journal:Br J Cancer
Journal Volume:92
Article Pages:72-9
Journal ISSN:1532-1827
Article Chemical List:Cell Extracts;Cytokines;Immunodominant Epitopes;Peptides;Receptor, erbB-2
Article MeSH List:Adoptive Transfer; Animals; Cell Extracts; Cell Line, Tumor; Cytokines(biosynthesis ); Cytotoxicity, Immunologic; Female; Humans; Immunodominant Epitopes(immunology ); Mice; Mice, SCID; Neoplasm Transplantation; Ovarian Neoplasms(immunology; metabolism ); Peptides(immunology ); Receptor, erbB-2(metabolism ); Research Support, Non-U.S. Gov't; T-Lymphocytes, Cytotoxic(immunology ); Transplantation, Heterologous; Tumor Cells, Cultured
Curation Last Updated:2009-11-09 19:30:51