Epitopes described in "A novel HLA-A*3303-restricted minor histocompatibility antigen encoded by an unconventional open reading frame of human TMSB4Y gene."

Article Authors:Hiroki Torikai; Yoshiki Akatsuka; Mikinori Miyazaki; Edus H Warren 3rd; Taku Oba; Kunio Tsujimura; Kazuo Motoyoshi; Yasuo Morishima; Yoshihisa Kodera; Kiyotaka Kuzushima; Toshitada Takahashi
Article Title:A novel HLA-A*3303-restricted minor histocompatibility antigen encoded by an unconventional open reading frame of human TMSB4Y gene.
Reference Detail
Reference ID:1012987
Abstract:Female-to-male hemopoietic stem cell transplantation (HSCT) elicits T cell responses against male-specific minor histocompatibility (H-Y) Ags encoded by the Y chromosome. All previously identified H-Y Ags are encoded by conventional open reading frames, but we report in this study the identification of a novel H-Y Ag encoded in the 5'-untranslated region of the TMSB4Y gene. An HLA-A*3303-restricted CD8(+) CTL clone was isolated from a male patient after an HSCT from his HLA-identical sister. Using a panel of cell lines carrying Y chromosome terminal deletions, a narrow region controlling the susceptibility of these target cells to CTL recognition was localized. Minigene transfection and epitope reconstitution assays identified an 11-mer peptide, EVLLRPGLHFR, designated TMSB4Y/A33, whose first amino acid was located 405 bp upstream of the TMSB4Y initiation codon. Analysis of the precursor frequency of CTL specific for recipient minor histocompatibility Ags in post-HSCT peripheral blood T cells revealed that a significant fraction of the total donor CTL response in this patient was directed against the TMSB4Y epitope. Tetramer analysis continued to detect TMSB4Y/A33-specific CD8(+) T cells at least up to 700 days post-HSCT. This finding underscores the in vivo immunological relevance of minor histocompatibility Ags derived from unconventional open reading frame products.
Affiliations:Division of Immunology, Aichi Cancer Center Research Institute, Aichi Cancer Center Hospital, Nagoya, Japan.
Reference Type:Literature
PubMed ID:15557202
Journal:J Immunol
Journal Volume:173
Article Pages:7046-54
Journal ISSN:0022-1767
Article Chemical List:5' Untranslated Regions;H-Y Antigen;HLA-A Antigens;HLA-A*33 antigen;Minor Histocompatibility Antigens;Protein Isoforms;RNA, Messenger;Thymosin;thymosin beta(4)
Article MeSH List:5' Untranslated Regions(chemistry; genetics; immunology); Amino Acid Sequence; Antigen Presentation(immunology); Base Sequence; Cell Line; Cell Line, Transformed; Chromosome Mapping; Chromosomes, Human, Y; Clone Cells; Cytotoxicity Tests, Immunologic; Female; H-Y Antigen(immunology; metabolism); HLA-A Antigens(genetics; immunology; metabolism); Hematopoietic Stem Cell Transplantation; Humans; Male; Middle Aged; Minor Histocompatibility Antigens(genetics; immunology; metabolism); Molecular Sequence Data; Open Reading Frames(immunology); Organ Specificity(genetics; immunology); Protein Isoforms(biosynthesis; genetics; immunology); RNA, Messenger(biosynthesis); Sequence Deletion(immunology); T-Lymphocytes, Cytotoxic(chemistry; immunology); Thymosin(biosynthesis; genetics; immunology)
Curation Last Updated:2014-10-03 21:12:28