Epitopes described in "IgG antibodies that recognize epitope Gly40-Arg43 in domain I of beta 2-glycoprotein I cause LAC, and their presence correlates strongly with thrombosis."

Article Authors:Bas de Laat; Ronald H W M Derksen; Rolf T Urbanus; Philip G de Groot
Article Title:IgG antibodies that recognize epitope Gly40-Arg43 in domain I of beta 2-glycoprotein I cause LAC, and their presence correlates strongly with thrombosis.
Reference Detail
Reference ID:1017422
Abstract:Anti-beta(2)-glycoprotein I antibodies are known to have a heterogeneous reactivity against beta(2)-glycoprotein I. We performed this study to characterize the epitope on beta(2)-glycoprotein I to which pathologic anti-beta(2)-glycoprotein I antibodies are directed. Plasma samples from 198 patients with various systemic autoimmune diseases were tested for the presence of lupus anticoagulant and anti-beta(2)-glycoprotein I immunoglobulin G (IgG) antibodies. The reactivity of the anti-beta(2)-glycoprotein I-positive samples was further tested by coating recombinant full-length beta(2)-glycoprotein I and 8 deletion mutants of beta(2)-glycoprotein I onto hydrophilic and hydrophobic enzyme-linked immunosorbent assay (ELISA) plates. Full-length beta(2)-glycoprotein I with point mutations in domain I at positions 8, 40, and 43 were used in inhibition experiments. Fifty-two patients with anti-beta(2)-glycoprotein I IgG antibodies could be divided into 2 patterns. Type A antibodies only recognize domain I when coated onto hydrophobic plates; they do not recognize domain I coated onto hydrophilic plates. Type B antibodies have heterogeneous reactivity for all domains. Type A antibodies recognize the epitope around amino acids Gly40-Arg43 and cause lupus anticoagulant activity. In contrast to type B antibodies, those of type A strongly correlated with thrombosis. In conclusion, antibodies directed at domain I (epitope comprising Gly40 and Arg43) have lupus anticoagulant activity and strongly associate with thrombosis.
Reference Type:Literature
PubMed ID:15507529
Journal Volume:105
Article Pages:1540-5
Journal ISSN:1528-0020
Article Chemical List:Epitopes;Glycoproteins;Immunoglobulin G;Lupus Coagulation Inhibitor;beta 2-Glycoprotein I;Arginine;Glycine
Article MeSH List:Adult; Antibody Specificity; Arginine; Binding Sites, Antibody; Enzyme-Linked Immunosorbent Assay; Epitopes(blood; immunology; metabolism); Female; Glycine; Glycoproteins(blood; immunology; metabolism); Humans; Immunoglobulin G(blood; metabolism); Lupus Coagulation Inhibitor(blood; immunology; metabolism); Male; Odds Ratio; Protein Structure, Tertiary; Thrombosis(blood; immunology); beta 2-Glycoprotein I
Curation Last Updated:2014-10-03 21:51:21