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| Article Authors: | Hiroaki Taguchi; Zhenyong Keck; Steven K H Foung; Sudhir Paul; Yasuhiro Nishiyama |
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| Article Title: | Antibody light chain-catalyzed hydrolysis of a hepatitis C virus peptide. |
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| Reference ID: | 1001389 |
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| Abstract: | A panel of human monoclonal and recombinant antibody light chains was screened for cleavage of the synthetic peptide corresponding to a neutralizing epitope of hepatitis C virus (residues 192-205 of envelope glycoprotein E1). One of the 39 light chains studied hydrolyzed the Val197-Ser198 bond of the peptide with Km and kcat values of 223 +/- 7 microM and 0.087 +/- 0.001 min(-1). |
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| Affiliations: | Chemical Immunology and Therapeutics Research Center, Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, 6431 Fannin, 77030, USA. |
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| Date: | 2004 |
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| Reference Type: | Literature |
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| PubMed ID: | 15357986 |
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| Journal: | Bioorg Med Chem Lett |
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| Journal Volume: | 14 |
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| Article Pages: | 4529-32 |
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| Journal ISSN: | 1464-3405 |
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| Article Chemical List: | Antibodies, Monoclonal;Hepatitis C Antibodies;Immunoglobulin Light Chains;Peptide Fragments;Peptide Library;Recombinant Proteins;Viral Proteins |
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| Article MeSH List: | Antibodies, Monoclonal(metabolism); Catalysis; Hepacivirus(immunology; metabolism); Hepatitis C Antibodies(metabolism); Humans; Hydrolysis; Immunoglobulin Light Chains(metabolism); Peptide Fragments(immunology; metabolism); Peptide Library; Recombinant Proteins(metabolism); Viral Proteins(immunology; metabolism) |
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| Curation Last Updated: | 2011-10-24 21:00:32 |
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