Epitopes described in "Species-specific immune response to immunization with human versus rodent A beta peptide."

Article Authors:Timothy J Seabrook; Jeanne K Bloom; Melitza Iglesias; Edward T Spooner; Dominic M Walsh; Cynthia A Lemere
Article Title:Species-specific immune response to immunization with human versus rodent A beta peptide.
Reference Detail
Reference ID:1014389
Abstract:Amyloid beta (A beta) immunization of amyloid precursor protein (APP)-transgenic (tg) mice with human A beta induces humoral immunity, however, the immune response to endogenous rodent A beta is unknown. Fourteen-month J20 APP-tg mice and non-tg littermates were immunized subcutaneously followed by chronic intranasal boosting with human or rodent A beta peptide and adjuvant LT(R192G). Rodent A beta-immunized APP-tg mice had anti-rodent A beta antibody levels of 257.8 micrograms/ml and those immunized with human A beta had anti-human A beta antibodies of 120.8 micrograms/ml. Non-tg littermates had anti-rodent and anti-human A beta antibody concentrations of 98.8 and 231.1 microgram/ml, respectively. Inter-species cross-reactivity was minimal. Anti-human A beta antibodies were predominately IgG1 and IgG2b, while anti-rodent A beta antibodies were equally IgG1, IgG2a, and IgG2b. Anti-human A beta antibodies recognized an epitope within human A beta1-9. Anti-rodent A beta antibodies did not stain Alzheimer's disease (AD) plaques but bound some plaques in APP-tg mice. Splenocytes proliferated modestly to their respective antigen and secreted low levels of IL-2 and IFN-gamma. Therefore, immunizing APP-tg and non-tg mice with rodent A beta resulted in a species-specific humoral response with modest T cell reactivity.
Affiliations:Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Reference Type:Literature
PubMed ID:15312960
Journal:Neurobiol Aging
Journal Volume:25
Article Pages:1141-51
Journal ISSN:0197-4580
Article Chemical List:Amyloid beta-Peptides;Antibodies;Epitopes;Immunoglobulin G;Interleukin-2;Interferon-gamma
Article MeSH List:Alzheimer Disease(immunology; physiopathology); Amyloid beta-Peptides(immunology); Animals; Animals, Genetically Modified; Antibodies(blood; immunology); Antibody Formation(genetics; immunology); Cells, Cultured; Disease Models, Animal; Epitopes(immunology); Female; Humans; Immunization; Immunoglobulin G(blood; immunology); Interferon-gamma(immunology); Interleukin-2(immunology); Male; Mice; Plaque, Amyloid(immunology); Species Specificity; T-Lymphocytes(immunology)
Curation Last Updated:2015-07-30 20:29:35