Epitopes described in "Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles."

Reference
Article Authors:Homayoun Shams; Peter Klucar; Steven E Weis; Ajit Lalvani; Patrick K Moonan; Hassan Safi; Benjamin Wizel; Katie Ewer; Gerald T Nepom; David M Lewinsohn; Peter Andersen; Peter F Barnes
Article Title:Characterization of a Mycobacterium tuberculosis peptide that is recognized by human CD4+ and CD8+ T cells in the context of multiple HLA alleles.
Reference Detail
Reference ID:565
Abstract:The secreted Mycobacterium tuberculosis 10-kDa culture filtrate protein (CFP)10 is a potent T cell Ag that is recognized by a high percentage of persons infected with M. tuberculosis. We determined the molecular basis for this widespread recognition by identifying and characterizing a 15-mer peptide, CFP10(71-85), that elicited IFN-gamma production and CTL activity by both CD4(+) and CD8(+) T cells from persons expressing multiple MHC class II and class I molecules, respectively. CFP10(71-85) contained at least two epitopes, one of 10 aa (peptide T1) and another of 9 aa (peptide T6). T1 was recognized by CD4(+) cells in the context of DRB1*04, DR5*0101, and DQB1*03, and by CD8(+) cells of A2(+) donors. T6 elicited responses by CD4(+) cells in the context of DRB1*04 and DQB1*03, and by CD8(+) cells of B35(+) donors. Deleting a single amino acid from the amino or carboxy terminus of either peptide markedly reduced IFN-gamma production, suggesting that they are minimal epitopes for both CD4(+) and CD8(+) cells. As far as we are aware, these are the shortest microbial peptides that have been found to elicit responses by both T cell subpopulations. The capacity of CFP10(71-85) to stimulate IFN-gamma production and CTL activity by CD4(+) and CD8(+) cells from persons expressing a spectrum of MHC molecules suggests that this peptide is an excellent candidate for inclusion in a subunit antituberculosis vaccine.
Affiliations:Center for Pulmonary and Infectious Disease Control, and Department of Microbiology, University of Texas Health Center, Tyler, TX 75708, USA. amir.shams@uthct.edu
Date:2004
Reference Type:Literature
PubMed ID:15265931
Journal:J Immunol
Journal Volume:173
Article Pages:1966-77
Journal ISSN:0022-1767
Article Chemical List:Antigens, Bacterial;Bacterial Proteins;CFP-10 protein (71-85), Mycobacterium tuberculosis;CFP-10 protein, Mycobacterium tuberculosis;Epitopes;HLA Antigens;HLA-DQ Antigens;HLA-DQ beta-Chains;HLA-DQB1 antigen;HLA-DR Antigens;HLA-DRB1 Chains;Peptide Fragments;Tuberculosis Vaccines;Interferon-gamma
Article MeSH List:Alleles; Amino Acid Substitution; Antigens, Bacterial(immunology); Bacterial Proteins(chemistry; immunology); CD4-Positive T-Lymphocytes(immunology; metabolism); CD8-Positive T-Lymphocytes(immunology; metabolism); Cytotoxicity, Immunologic; Epitopes(chemistry; immunology); HLA Antigens(immunology); HLA-DQ Antigens(immunology); HLA-DQ beta-Chains; HLA-DR Antigens(immunology); HLA-DRB1 Chains; Humans; Interferon-gamma(biosynthesis); Mycobacterium tuberculosis(immunology); Peptide Fragments(chemistry; immunology); Sequence Deletion; T-Cell Antigen Receptor Specificity; Tuberculosis Vaccines
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