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| Article Authors: | Colleen Olive; Michael Batzloff; Aniko Horváth; Timothy Clair; Penny Yarwood; Istvan Toth; Michael F Good |
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| Article Title: | Group A streptococcal vaccine delivery by immunization with a self-adjuvanting M protein-based lipid core peptide construct. |
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| Reference ID: | 1013308 |
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| Abstract: | BACKGROUND & OBJECTIVES: To develop a broad strain coverage GAS vaccine, several strategies have been investigated which included multi-epitope approaches as well as targeting the M protein conserved Cregion. These approaches, however, have relied on the use of adjuvants that are toxic for human application. The development of safe and effective adjuvants for human use is a key issue in the development of effective vaccines. In this study, we investigated the lipid polylysine core peptide (LCP) system as a self-adjuvanting GAS vaccine delivery approach. METHODS: An LCP-GAS construct was synthesised incorporating multiple copies of a protective peptide epitope (J8) from the conserved carboxy terminal C-repeat region of the M protein. B10.BR mice were immunized parenterally with the LCP-J8 construct, with or without conventional adjuvant, prior to the assessment of immunogenicity and the induction of serum opsonic antibodies. RESULTS: Our data demonstrated immunogenicity of LCP-J8 when coadministered in complete Freund's adjuvant (CFA), or administered in the absence of conventional adjuvant. In both cases, immunization led to the induction of high-titre J8 peptide-specific serum IgG antibody responses, and the induction of heterologous opsonic antibodies that did not cross-react with human heart tissue proteins. INTERPRETATION & CONCLUSION: These data indicated the potential of a novel self-adjuvanting LCP vaccine delivery system incorporating a synthetic GAS M protein C-region peptide immunogen in the induction of broadly protective immune responses, and pointed to the potential application of this system in human vaccine development against infectious diseases. |
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| Affiliations: | Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research PO Royal Brisbane Hospital, University of Queensland, Brisbane Queensland, Australia. colleenO@qimr.edu.au |
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| Date: | 2004 |
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| Reference Type: | Literature |
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| PubMed ID: | 15232170 |
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| Journal: | Indian J Med Res |
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| Journal Volume: | 119 Suppl |
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| Article Pages: | 88-94 |
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| Journal ISSN: | 0971-5916 |
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| Article Chemical List: | Antibodies, Bacterial;Bacterial Proteins;Bacterial Vaccines;Lipids;Peptides |
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| Article MeSH List: | Amino Acid Sequence; Antibodies, Bacterial(biosynthesis ); Bacterial Proteins(chemistry ); Bacterial Vaccines(administration & dosage; chemistry; immunology ); Blotting, Western; Enzyme-Linked Immunosorbent Assay; Lipids(chemistry ); Molecular Sequence Data; Peptides(chemistry ); Streptococcus(immunology ) |
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| Curation Last Updated: | 2013-05-28 21:31:08 |
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