Epitopes described in "An exposed domain in the severe acute respiratory syndrome coronavirus spike protein induces neutralizing antibodies."

Article Authors:Tong Zhou; Hong Wang; Danlin Luo; Thomas Rowe; Zheng Wang; Robert J Hogan; Shihong Qiu; Robert J Bunzel; Guoqiang Huang; Vinod Mishra; Thomas G Voss; Robert Kimberly; Ming Luo
Article Title:An exposed domain in the severe acute respiratory syndrome coronavirus spike protein induces neutralizing antibodies.
Reference Detail
Reference ID:320
Abstract:Exposed epitopes of the spike protein may be recognized by neutralizing antibodies against severe acute respiratory syndrome (SARS) coronavirus (CoV). A protein fragment (S-II) containing predicted epitopes of the spike protein was expressed in Escherichia coli. The properly refolded protein fragment specifically bound to the surface of Vero cells. Monoclonal antibodies raised against this fragment recognized the native spike protein of SARS CoV in both monomeric and trimeric forms. These monoclonal antibodies were capable of blocking S-II attachment to Vero cells and exhibited in vitro antiviral activity. These neutralizing antibodies mapped to epitopes in two peptides, each comprising 20 amino acids. Thus, this region of the spike protein might be a target for generation of therapeutic neutralizing antibodies against SARS CoV and for vaccine development to elicit protective humoral immunity.
Affiliations:Department of Medicine, University of Alabama at Birmingham, 35294, USA.
Reference Type:Literature
PubMed ID:15194798
Journal:J Virol
Journal Volume:78
Article Pages:7217-26
Journal ISSN:1098-5514
Article Chemical List:Antibodies, Monoclonal;Antibodies, Viral;Membrane Glycoproteins;Viral Envelope Proteins;Viral Vaccines;spike glycoprotein, coronavirus
Article MeSH List:Amino Acid Sequence; Animals; Antibodies, Monoclonal(biosynthesis; immunology); Antibodies, Viral(biosynthesis; blood; immunology); Cercopithecus aethiops; Epitope Mapping; Escherichia coli(genetics; metabolism); Female; Humans; Immunization; Membrane Glycoproteins(chemistry; genetics; immunology); Mice; Mice, Inbred BALB C; Molecular Sequence Data; Neutralization Tests; Plaque Assay; SARS Virus(immunology); Severe Acute Respiratory Syndrome(prevention & control; virology); Vero Cells; Viral Envelope Proteins(chemistry; genetics; immunology); Viral Vaccines(administration & dosage; immunology)
Curation Last Updated:2011-08-30 20:02:10