Epitopes described in "Tracking the recruitment of diabetogenic CD8+ T-cells to the pancreas in real time."

Article Authors:Anna Moore; Jan Grimm; Bingye Han; Pere Santamaria
Article Title:Tracking the recruitment of diabetogenic CD8+ T-cells to the pancreas in real time.
Reference Detail
Reference ID:1007488
Abstract:Development of autoimmune diabetes in both humans and mice is preceded by a prolonged period of inflammation of pancreatic islets by autoreactive T-cells. Noninvasive imaging techniques, including positron-emission tomography and optical or magnetic resonance imaging, have been used to track the recruitment of lymphocytes to sites of inflammation. These techniques, however, rely on labeling strategies that are non-antigen specific and do not allow specific tracking of the recruitment of autoreactive lymphocytes. Here we describe an antigen-specific magnetic label to selectively target a prevalent population of diabetogenic CD8(+) T-cells that contribute to the progression of insulitis to overt diabetes in NOD mice. Superparamagnetic nanoparticles coated with multiple copies of a high-avidity peptide/major histocompatibility complex ligand of these T-cells (NRP-V7/K(d)) are endocytosed by CD8(+) T-cells in an antigen-specific manner. Using these T-cells as probes, we show that inflammation of pancreatic islets by autoreactive T-cells can be detected in real time by magnetic resonance imaging. This study demonstrates the feasibility of visualizing the presence of ongoing autoimmune responses noninvasively.
Reference Type:Literature
PubMed ID:15161749
Journal Volume:53
Article Pages:1459-66
Journal ISSN:0012-1797
Article Chemical List:Ligands;Oxides;Peptides;Iron
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(pathology); Computer Systems; Diabetes Mellitus, Type 1(diagnosis; etiology; pathology; physiopathology); Endocytosis; Iron(diagnostic use); Ligands; Magnetic Resonance Imaging; Mice; Mice, Inbred NOD; Microscopy, Fluorescence; Nanotubes; Oxides(diagnostic use); Pancreas(pathology; physiopathology); Peptides(diagnostic use)
Curation Last Updated:2015-07-30 20:20:54