Epitopes described in "Microarray immunoassay: association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes."

Article Authors:Wayne G Shreffler; Kirsten Beyer; Te-Hua Tearina Chu; A Wesley Burks; Hugh A Sampson
Article Title:Microarray immunoassay: association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes.
Reference Detail
Reference ID:1014739
Abstract:BACKGROUND: IgE epitope mapping of food allergens is a prerequisite for engineering hypoallergenic immunotherapeutic agents and might reveal basic information regarding a patient's immune response. Mapping of large numbers of epitopes by using individual patient sera has been impractical with current techniques. OBJECTIVE: We sought to develop a peptide microarray-based immunoassay to map peanut epitopes by using microliter quantities of serum. METHODS: A set of 213 overlapping 20-residue peptides was synthesized corresponding to the primary sequences of Ara h 1, Ara h 2, and Ara h 3. These were arrayed in triplicate along with the corresponding recombinant proteins onto glass slides and used for immunolabeling. RESULTS: Seventy-seven patient and 15 control sera were analyzed. The majority of patients (97%) had specific IgE to at least one of the recombinant allergens, and 87% had detectable IgE to sequential epitopes. Microarray mapping correlated well with previous studies. However, the analysis of individual patients revealed remarkable heterogeneity in the number and patterns of epitope recognition. High epitope diversity was found in patients with a history of more severe allergic reactions. Also, sensitization of effector cells with more diverse IgE antibodies conferred greater reactivity to specific allergen. CONCLUSIONS: The protein microarray immunoassay confirmed that Ara h 1, Ara h 2, and Ara h 3 are major peanut allergens and allows for parallel epitope analysis. This has led to the discovery of an additional important epitope of Ara h 1 and the recognition of a high degree of patient heterogeneity. This qualitative difference in epitope diversity might provide prognostic information about the patient.
Affiliations:Jaffe Food Allergy Institute, Division of Allergy and Immunology, Department of Pediatrics, Mount Sinai Medical Center, Box 1198, One Gustave L. Levy Place, New York, NY 10029, USA.
Reference Type:Literature
PubMed ID:15100687
Journal:J Allergy Clin Immunol
Journal Volume:113
Article Pages:776-82
Journal ISSN:0091-6749
Article Chemical List:2S Albumins, Plant;Allergens;Antigens, Plant;Ara h 1 protein, Arachis hypogaea;Ara h II protein, Arachis hypogaea;Epitopes;Glycoproteins;Plant Proteins;Recombinant Proteins;Seed Storage Proteins;allergen Ara h3;Immunoglobulin E
Article MeSH List:2S Albumins, Plant; Adolescent; Adult; Allergens(immunology); Antigens, Plant; Child; Child, Preschool; Epitopes; Glycoproteins(immunology); Humans; Immunoassay; Immunoglobulin E(immunology); In Vitro Techniques; Infant; Oligonucleotide Array Sequence Analysis; Peanut Hypersensitivity(immunology; physiopathology); Plant Proteins(immunology); Recombinant Proteins(immunology); Seed Storage Proteins; Severity of Illness Index
Curation Last Updated:2015-06-05 01:39:40