Epitopes described in "Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120."

Article Authors:Chih-chin Huang; Miro Venturi; Shahzad Majeed; Michael J Moore; Sanjay Phogat; Mei-Yun Zhang; Dimiter S Dimitrov; Wayne A Hendrickson; James Robinson; Joseph Sodroski; Richard Wyatt; Hyeryun Choe; Michael Farzan; Peter D Kwong
Article Title:Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120.
Reference Detail
Reference ID:1086
Abstract:The conserved surface of the HIV-1 gp120 envelope glycoprotein that binds to the HIV-1 coreceptor is protected from humoral recognition by multiple layers of camouflage. Here we present sequence and genomic analyses for 12 antibodies that pierce these defenses and determine the crystal structures of 5. The data reveal mechanisms and atomic-level details for three unusual immune features: posttranslational mimicry of coreceptor by tyrosine sulfation of antibody, an alternative molecular mechanism controlling such sulfation, and highly selective V(H)-gene usage. When confronted by extraordinary viral defenses, the immune system unveils novel adaptive capabilities, with tyrosine sulfation enhancing the vocabulary of antigen recognition.
Affiliations:Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Reference Type:Literature
PubMed ID:14981267
Journal:Proc Natl Acad Sci U S A
Journal Volume:101
Article Pages:2706-11
Journal ISSN:0027-8424
Article Chemical List:Antigens, CD4;HIV Envelope Protein gp120;Immunoglobulin Fab Fragments;Immunoglobulin Heavy Chains;Immunoglobulin Joining Region;Immunoglobulin Variable Region;Sulfates;Tyrosine;tyrosine O-sulfate
Article MeSH List:Acquired Immunodeficiency Syndrome(immunology); Amino Acid Sequence; Antibody Formation; Antigens, CD4(immunology); Crystallography, X-Ray; Genes, Immunoglobulin; HIV Envelope Protein gp120(immunology); HIV Infections(immunology); HIV-1(immunology); Humans; Immunoglobulin Fab Fragments(chemistry); Immunoglobulin Heavy Chains(genetics); Immunoglobulin Joining Region(chemistry; genetics); Immunoglobulin Variable Region(chemistry; genetics); Molecular Sequence Data; Sulfates(metabolism); Tyrosine(analogs & derivatives; metabolism)
Curation Last Updated:2015-06-05 00:14:08