Epitopes described in "A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects."

Reference
Article Authors:Markus G Rudolph; Lucy Q Shen; Stephen A Lamontagne; John G Luz; Joseph R Delaney; Qing Ge; Bryan K Cho; Deborah Palliser; Carol A McKinley; Jianzhu Chen; Ian A Wilson; Herman N Eisen
Article Title:A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects.
Reference Detail
Reference ID:1002486
Abstract:We identify and consider some characteristics of a peptide antagonist for the Ag-specific receptor on 2C cells (the 2C TCR). The peptide, GNYSFYAL (called GNY), binds to H-2K(b), and a very high-resolution crystal structure of the GNY-K(b) complex at 1.35 A is described. Although the GNY peptide does not bind to L(d), the potency of GNY-K(b) as an antagonist is evident from its ability to specifically inhibit 2C TCR-mediated reactions to an allogenic agonist complex (QLSPFPFDL-L(d)), as well as to a syngeneic agonist complex (SIYRYYGL-K(b)). The crystal structure and the activities of alanine-substituted peptide variants point to the properties of the peptide P4 side chain and the conformation of the Tyr-P6 side chain as the structural determinants of GNYSFYAL antagonist activity.
Date:2004
Reference Type:Literature
PubMed ID:14978103
Journal:J Immunol
Journal Volume:172
Article Pages:2994-3002
Journal ISSN:0022-1767
Article Chemical List:H-2 Antigens;H-2Kb protein, mouse;Histocompatibility Antigen H-2D;Isoantigens;Oligopeptides;Receptors, Antigen, T-Cell;Serine;Arginine;Lysine;Alanine
Article MeSH List:Alanine(metabolism); Amino Acid Sequence; Amino Acid Substitution(immunology); Animals; Arginine(metabolism); Cell Line; Cell Line, Tumor; Clone Cells; Crystallography, X-Ray; Cytotoxicity Tests, Immunologic; Female; H-2 Antigens(metabolism); Histocompatibility Antigen H-2D; Isoantigens(physiology); Lysine(metabolism); Mice; Mice, Inbred C57BL; Mice, Transgenic; Oligopeptides(chemistry; metabolism; physiology); Protein Binding(immunology); Receptors, Antigen, T-Cell(agonists; antagonists & inhibitors; physiology); Serine(metabolism); Structure-Activity Relationship
Curation Last Updated:2014-10-03 20:19:10