Epitopes described in "Promiscuity of MHC class Ib-restricted T cell responses."

Article Authors:Alexander Ploss; Gregoire Lauvau; Brian Contos; Kristen M Kerksiek; Patrick D Guirnalda; Ingrid Leiner; Laurel L Lenz; Michael J Bevan; Eric G Pamer
Article Title:Promiscuity of MHC class Ib-restricted T cell responses.
Reference Detail
Reference ID:1219
Abstract:Murine infection with the Gram-positive intracellular bacterium Listeria monocytogenes activates CD8(+) T cells that recognize bacterially derived N-formyl methionine peptides in the context of H2-M3 MHC class Ib molecules. Three peptides, fMIGWII, fMIVIL, and fMIVTLF, are targets of L. monocytogenes-specific CD8(+) T cells. To investigate epitope cross-recognition by H2-M3-restricted CD8(+) T cells, we deleted the sequence encoding fMIGWII from a virulent strain of L. monocytogenes. Infection with fMIGWII-deficient L. monocytogenes unexpectedly primed CD8(+) T cells that stain with fMIGWII/H2-M3 tetramers and lyse fMIGWII-coated target cells in vivo. Because the fMIGWII sequence is nonredundant, we speculated that other bacterially derived Ags are priming these responses. HPLC peptide fractionation of bacterial culture supernatants revealed several distinct L. monocytogenes-derived peptides that are recognized by fMIGWII-specific T cells. Our results demonstrate that the dominant H2-M3-restricted CD8(+) T cell population, although reactive with fMIGWII, is primed by other, non-fMIGWII peptides derived from L. monocytogenes. Although this degree of Ag receptor promiscuity is unusual for the adaptive immune system, it may be a more common feature of T cell responses restricted by nonpolymorphic MHC class Ib molecules.
Affiliations:Infectious Diseases Service, Department of Medicine and Laboratory of Antimicrobial Immunity, Immunology Program, Sloan-Kettering Institute, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Reference Type:Literature
PubMed ID:14634106
Journal:J Immunol
Journal Volume:171
Article Pages:5948-55
Journal ISSN:0022-1767
Article Chemical List:Epitopes, T-Lymphocyte;H2-M3 antigen;Histocompatibility Antigens Class I;Immunodominant Epitopes;Ligands
Article MeSH List:Animals; CD8-Positive T-Lymphocytes(immunology; metabolism; microbiology); Cell Line; Cell Line, Tumor; Clone Cells; Cytotoxicity, Immunologic; Epitopes, T-Lymphocyte(genetics; immunology; metabolism); Female; Histocompatibility Antigens Class I(genetics; immunology; metabolism); Immunodominant Epitopes(genetics; immunology; metabolism); Injections, Intravenous; Ligands; Listeria monocytogenes(genetics; immunology); Mast-Cell Sarcoma(immunology); Mice; Mice, Inbred C57BL; Sequence Deletion; T-Lymphocyte Subsets(immunology; metabolism; microbiology); T-Lymphocytes, Cytotoxic(immunology; metabolism; microbiology)
Curation Last Updated:2015-07-30 20:02:37