Epitopes described in "Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b)."

Article Authors:Julie Dam; Rongjin Guan; Kannan Natarajan; Nazzareno Dimasi; Lukasz K Chlewicki; David M Kranz; Peter Schuck; David H Margulies; Roy A Mariuzza
Article Title:Variable MHC class I engagement by Ly49 natural killer cell receptors demonstrated by the crystal structure of Ly49C bound to H-2K(b).
Reference Detail
Reference ID:1140
Abstract:The Ly49 family of natural killer (NK) receptors regulates NK cell function by sensing major histocompatibility complex (MHC) class I. Ly49 receptors show complex patterns of MHC class I cross-reactivity and, in certain cases, peptide selectivity. To investigate whether specificity differences result from topological differences in MHC class I engagement, we determined the structure of the peptide-selective receptor Ly49C in complex with H-2K(b). The Ly49C homodimer binds two MHC class I molecules in symmetrical way, a mode distinct from that of Ly49A, which binds MHC class I asymmetrically. Ly49C does not directly contact the MHC-bound peptide. In addition, MHC crosslinking by Ly49C was demonstrated in solution. We propose a dynamic model for Ly49-MHC class I interactions involving conformational changes in the receptor, whereby variations in Ly49 dimerization mediate different MHC-binding modes.
Affiliations:Center for Advanced Research in Biotechnology, W.M. Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, Maryland 20850, USA.
Reference Type:Literature
PubMed ID:14595439
Journal:Nat Immunol
Journal Volume:4
Article Pages:1213-22
Journal ISSN:1529-2908
Article Chemical List:Antigens, Ly;H-2 Antigens;H-2Kb protein, mouse;Klra1 protein, mouse;Klra3 protein, mouse;Lectins, C-Type;NK Cell Lectin-Like Receptor Subfamily A;Receptors, NK Cell Lectin-Like
Article MeSH List:Animals; Antigens, Ly(chemistry; physiology); Crystallography, X-Ray; Dimerization; H-2 Antigens(chemistry; physiology); Killer Cells, Natural(immunology); Lectins, C-Type; Major Histocompatibility Complex(physiology); NK Cell Lectin-Like Receptor Subfamily A; Protein Binding(immunology); Protein Structure, Quaternary; Receptors, NK Cell Lectin-Like; Substrate Specificity(physiology)
Curation Last Updated:2015-07-30 20:02:31