Epitopes described in "Pregnancy induces minor histocompatibility antigen-specific cytotoxic T cells: implications for stem cell transplantation and immunotherapy."

Article Authors:Rob M Verdijk; Antoinette Kloosterman; Jos Pool; Maarten van de Keur; Albert M I H Naipal; Astrid G S van Halteren; Anneke Brand; Tuna Mutis; Els Goulmy
Article Title:Pregnancy induces minor histocompatibility antigen-specific cytotoxic T cells: implications for stem cell transplantation and immunotherapy.
Reference Detail
Reference ID:1012981
Abstract:Recipients of HLA-identical stem cell transplants have a poorer transplant outcome if the donor is female rather than male. We analyzed whether pregnancy primes for minor histocompatibility (H) antigens. Peripheral blood mononuclear cells (PBMCs) from healthy multiparous female blood donors were depleted for CD4+, CD14+, CD16+, and CD19+ cells, stained with minor H antigen-specific HLA-A2 tetramers, sorted by fluorescence-activated cell sorting, and tested for cytotoxic activity. Minor H antigens HY-, HA-1-, and HA-2-specific cytotoxic T cells (CD8+, CD45RA-) were present in PBMCs from 4 of 7 female donors up to 22 years after the last delivery. Interestingly, in 2 of the 4 cases microchimerism of the putative immunizing minor H antigen was observed. Thus, pregnancy can lead to alloimmune responses against the infant's paternal minor H antigens. The minor H antigen immunization status of female donors raises important questions for the clinical practice of stem cell transplantation.
Affiliations:Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Reference Type:Literature
PubMed ID:14592836
Journal Volume:103
Article Pages:1961-4
Journal ISSN:0006-4971
Article Chemical List:Antibodies, Monoclonal;Antigens, CD14;Antigens, CD19;Antigens, CD4;HLA-A2 Antigen;Histocompatibility Antigens Class I;Minor Histocompatibility Antigens;Peptides;Receptors, IgG
Article MeSH List:Antibodies, Monoclonal(blood ); Antigens, CD14(biosynthesis ); Antigens, CD19(biosynthesis ); Antigens, CD4(biosynthesis ); Blood Donors; Cell Separation; Female; Flow Cytometry; HLA-A2 Antigen(biosynthesis ); Histocompatibility; Histocompatibility Antigens Class I(chemistry ); Humans; Immunologic Memory; Immunotherapy(methods ); Leukocytes, Mononuclear(metabolism ); Male; Minor Histocompatibility Antigens(metabolism ); Peptides(chemistry ); Pregnancy; Receptors, IgG(biosynthesis ); Reverse Transcriptase Polymerase Chain Reaction; Stem Cell Transplantation(methods ); T-Lymphocytes, Cytotoxic(metabolism )
Curation Last Updated:2014-10-03 21:12:27