Epitopes described in "Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis."

Article Authors:Homayoun Shams; Peter F Barnes; Stephen E Weis; Peter Klucar; Benjamin Wizel
Article Title:Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis.
Reference Detail
Reference ID:788
Abstract:Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptides that elicited cytolytic T lymphocyte activity and interferon-gamma production by CD8+ T cells from HLA-A*0201+ healthy tuberculin reactors. These peptides were derived from the 38-kDa antigen and the 28-kDa hemolysin, the latter being a novel target for CD8+ T cells. We speculate that hemolysins may alter the phagosomal membrane surrounding intracellular M. tuberculosis, allowing themselves and other antigens to gain access to the major histocompatibility complex class I processing pathway.
Affiliations:Center for Pulmonary and Infectious Disease Control, University of Texas Health Center, 11937 U.S. Hwy. 271, Tyler, TX 75708-3154, USA. amir.shams@uthct.edu
Reference Type:Literature
PubMed ID:12972510
Journal:J Leukoc Biol
Journal Volume:74
Article Pages:1008-14
Journal ISSN:0741-5400
Article Chemical List:Antigens, Bacterial;Epitopes, T-Lymphocyte;HLA-A Antigens;HLA-A*02:01 antigen;HLA-A2 Antigen;Hemolysin Proteins;Peptide Fragments;Mycobacterium tuberculosis antigens
Article MeSH List:Adult; Antigens, Bacterial(immunology; metabolism); CD8-Positive T-Lymphocytes(immunology); Enzyme-Linked Immunosorbent Assay; Epitopes, T-Lymphocyte(immunology); HLA-A Antigens(metabolism); HLA-A2 Antigen; Hemolysin Proteins(immunology); Humans; Molecular Weight; Mycobacterium tuberculosis(immunology); Peptide Fragments(metabolism); T-Lymphocytes, Cytotoxic(immunology)
Curation Last Updated:2014-10-03 19:35:41