Epitopes described in "Identification of novel immunodominant CD4+ Th1-type T-cell peptide epitopes from herpes simplex virus glycoprotein D that confer protective immunity."

Article Authors:Lbachir BenMohamed; Georges Bertrand; Cory D McNamara; Helene Gras-Masse; Juergen Hammer; Steven L Wechsler; Anthony B Nesburn
Article Title:Identification of novel immunodominant CD4+ Th1-type T-cell peptide epitopes from herpes simplex virus glycoprotein D that confer protective immunity.
Reference Detail
Reference ID:1004615
Abstract:The molecular characterization of the epitope repertoire on herpes simplex virus (HSV) antigens would greatly expand our knowledge of HSV immunity and improve immune interventions against herpesvirus infections. HSV glycoprotein D (gD) is an immunodominant viral coat protein and is considered an excellent vaccine candidate antigen. By using the TEPITOPE prediction algorithm, we have identified and characterized a total of 12 regions within the HSV type 1 (HSV-1) gD bearing potential CD4(+) T-cell epitopes, each 27 to 34 amino acids in length. Immunogenicity studies of the corresponding medium-sized peptides confirmed all previously known gD epitopes and additionally revealed four new immunodominant regions (gD(49-82), gD(146-179), gD(228-257), and gD(332-358)), each containing naturally processed epitopes. These epitopes elicited potent T-cell responses in mice of diverse major histocompatibility complex backgrounds. Each of the four new immunodominant peptide epitopes generated strong CD4(+) Th1 T cells that were biologically active against HSV-1-infected bone marrow-derived dendritic cells. Importantly, immunization of H-2(d) mice with the four newly identified CD4(+) Th1 peptide epitopes but not with four CD4(+) Th2 peptide epitopes induced a robust protective immunity against lethal ocular HSV-1 challenge. These peptide epitopes may prove to be important components of an effective immunoprophylactic strategy against herpes.
Affiliations:Department of Ophthalmology, University of California-Irvine, College of Medicine, Orange, California 92868, USA. Lbenmoha@uci.edu.
Reference Type:Literature
PubMed ID:12915561
Journal:J Virol
Journal Volume:77
Article Pages:9463-73
Journal ISSN:1098-5514
Article Chemical List:Antigens, Viral;Herpesvirus Vaccines;Immunodominant Epitopes;Interleukin-2;Viral Envelope Proteins;glycoprotein D, Human herpesvirus 1;Interferon-gamma
Article MeSH List:Algorithms; Amino Acid Sequence; Animals; Antigens, Viral(chemistry; genetics); Herpes Simplex(immunology; prevention & control); Herpesvirus 1, Human(genetics; immunology); Herpesvirus Vaccines(genetics; pharmacology); Immunization; Immunodominant Epitopes(chemistry; genetics); Interferon-gamma(biosynthesis); Interleukin-2(biosynthesis); Lymphocyte Depletion; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Molecular Sequence Data; Th1 Cells(immunology); Viral Envelope Proteins(genetics; immunology)
Curation Last Updated:2015-09-25 18:04:42