Epitopes described in "Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles."

Article Authors:Mayla Hsu; Jie Zhang; Mike Flint; Carine Logvinoff; Cecilia Cheng-Mayer; Charles M Rice; Jane A McKeating
Article Title:Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles.
Reference Detail
Reference ID:1004911
Abstract:HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
Reference Type:Literature
PubMed ID:12761383
Journal:Proc Natl Acad Sci U S A
Journal Volume:100
Article Pages:7271-6
Journal ISSN:1091-6490
Article Chemical List:Antibodies, Monoclonal;Antigens, CD;Antigens, CD81;CD81 protein, human;E1 protein, Hepatitis C virus;Hepatitis C Antibodies;Membrane Proteins;Receptors, Virus;Viral Envelope Proteins;glycoprotein E2, Hepatitis C virus
Article MeSH List:Antibodies, Monoclonal; Antigens, CD(physiology); Antigens, CD81; Cell Line; Chimera(genetics; immunology); HIV(genetics; pathogenicity; physiology); HeLa Cells; Hepacivirus(genetics; pathogenicity; physiology); Hepatitis C Antibodies; Hepatocytes(virology); Humans; Hydrogen-Ion Concentration; Membrane Proteins(antagonists & inhibitors; physiology); Neutralization Tests; Receptors, Virus(antagonists & inhibitors; physiology); Viral Envelope Proteins(chemistry; genetics; immunology; physiology)
Curation Last Updated:2014-10-03 20:36:50