Epitopes described in "Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles."

Article Authors:Mayla Hsu; Jie Zhang; Mike Flint; Carine Logvinoff; Cecilia Cheng-Mayer; Charles M Rice; Jane A McKeating
Article Title:Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles.
Reference Detail
Reference ID:1004911
Abstract:HIV pseudotypes bearing native hepatitis C virus (HCV) glycoproteins (strain H and Con1) are infectious for the human hepatoma cell lines Huh-7 and PLC/PR5. Infectivity depends on coexpression of both E1 and E2 glycoproteins, is pH-dependent, and can be neutralized by mAbs mapping to amino acids 412-447 within E2. Cell-surface expression of one or all of the candidate receptor molecules (CD81, low-density lipoprotein receptor, scavenger receptor class B type 1, and dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin) failed to confer permissivity to HIV-HCV pseudotype infection. However, HIV-HCV pseudotype infectivity was inhibited by a recombinant soluble form of CD81 and a mAb specific for CD81, suggesting that CD81 may be a component of a receptor complex.
Affiliations:Aaron Diamond AIDS Research Center and Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
Reference Type:Literature
PubMed ID:12761383
Journal:Proc Natl Acad Sci U S A
Journal Volume:100
Article Pages:7271-6
Journal ISSN:0027-8424
Article Chemical List:Antibodies, Monoclonal;Antigens, CD;Antigens, CD81;CD81 protein, human;E1 protein, Hepatitis C virus;Hepatitis C Antibodies;Membrane Proteins;Receptors, Virus;Viral Envelope Proteins;glycoprotein E2, Hepatitis C virus
Article MeSH List:Antibodies, Monoclonal; Antigens, CD(physiology); Antigens, CD81; Cell Line; Chimera(genetics; immunology); HIV(genetics; pathogenicity; physiology); HeLa Cells; Hepacivirus(genetics; pathogenicity; physiology); Hepatitis C Antibodies; Hepatocytes(virology); Humans; Hydrogen-Ion Concentration; Membrane Proteins(antagonists & inhibitors; physiology); Neutralization Tests; Receptors, Virus(antagonists & inhibitors; physiology); Viral Envelope Proteins(chemistry; genetics; immunology; physiology)
Curation Last Updated:2015-12-10 20:01:50