Epitopes described in "Celiac disease association with CD8+ T cell responses: identification of a novel gliadin-derived HLA-A2-restricted epitope."

Article Authors:Carmen Gianfrani; Riccardo Troncone; Patrizia Mugione; Elena Cosentini; Mariateresa De Pascale; Clementina Faruolo; Stefania Senger; Giuseppe Terrazzano; Scott Southwood; Salvatore Auricchio; Alessandro Sette
Article Title:Celiac disease association with CD8+ T cell responses: identification of a novel gliadin-derived HLA-A2-restricted epitope.
Reference Detail
Reference ID:200151
Abstract:One of the diagnostic hallmarks of the histological lesions associated with celiac disease is the extensive infiltration of the small intestinal epithelium by CD8(+) T cells of unknown Ag specificity. In this study, we report recognition of the gliadin-derived peptide (A-gliadin 123-132) by CD8(+) T lymphocytes from celiac patients. A-gliadin 123-132-specific IFN-gamma production and cytotoxic activity were detected in PBMCs derived from patients on gluten-free diet, but not from either celiac patients on gluten-containing diet or healthy controls. In contrast, A-gliadin 123-132-specific cells were isolated from small intestine biopsies of patients on either gluten-free or gluten-containing diets. Short-term T cell lines derived from the small intestinal mucosa and specific for the 123-132 epitope recognized human APC pulsed with either whole recombinant alpha-gliadin or a partial pepsin-trypsin gliadin digest. Finally, we speculate on a possible mechanism leading to processing and presentation of class I-restricted gliadin-derived epitopes in celiac disease patients.
Affiliations:Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche, Avellino, Italy. cgianfrani@isa.av.cnr.it
Reference Type:Literature
PubMed ID:12594302
Journal:J Immunol
Journal Volume:170
Article Pages:2719-26
Journal ISSN:0022-1767
Article Chemical List:Epitopes, T-Lymphocyte;HLA-A2 Antigen;Peptide Fragments;Gliadin
Article MeSH List:Adolescent; Adult; Antigen Presentation; CD8-Positive T-Lymphocytes(immunology; metabolism); Celiac Disease(blood; immunology; pathology); Cell Line; Cell Movement(immunology); Child; Child, Preschool; Cytotoxicity Tests, Immunologic; Epitopes, T-Lymphocyte(blood; immunology); Gliadin(blood; immunology); HLA-A2 Antigen(blood; immunology); Humans; Intestinal Mucosa(immunology; pathology); Peptide Fragments(blood; immunology); Protein Processing, Post-Translational(immunology); T-Lymphocytes, Cytotoxic(immunology; metabolism)
Curation Last Updated:2014-10-03 19:43:02