Epitopes described in "Vigorous innate and virus-specific cytotoxic T-lymphocyte responses to murine cytomegalovirus in the submaxillary salivary gland."

Article Authors:Victoria J Cavanaugh; Yuping Deng; Mark P Birkenbach; Jacquelyn S Slater; Ann E Campbell
Article Title:Vigorous innate and virus-specific cytotoxic T-lymphocyte responses to murine cytomegalovirus in the submaxillary salivary gland.
Reference Detail
Reference ID:1025825
Abstract:To better understand the immunological mechanisms that permit prolonged shedding of murine cytomegalovirus (MCMV) from the salivary gland, the phenotypic and functional characteristics of leukocytes infiltrating the submaxillary gland (SMG) were analyzed in infected BALB/c mice. A robust innate immune response, comprised of CD11c+ major histocompatibility complex class II+ CD11b- CD8alpha+ dendritic cells and gamma/delta T-cell receptor-bearing CD3+ T cells was prominent through at least 28 days postinfection. Concurrently, a dramatic increase in pan-NK (DX5+) CD3+ and CD8+ T cells was observed, while CD4+ T cells, known to be essential for viral clearance from this tissue, increased slightly. The expression particularly of gamma interferon but also of interleukin-10 and CC chemokines was extraordinarily high in the SMG in response to MCMV infection. The gamma interferon was produced primarily by CD4+ and CD8+ T lymphocytes and DX5+ CD3+ T cells. The SMG CD8+ T cells were highly cytolytic ex vivo, and a significant proportion of these cells were specific to an immunodominant MCMV peptide. These peptide-specific clones were not exhausted by the presence of high virus titers, which persisted in the SMG despite the strength of the cell-mediated responses. In contrast, MCMV replication was efficiently cleared from the draining cervical and periglandular lymph nodes, a tissue displaying a substantially weaker antiviral response. Our data indicated that vigorous innate and acquired immune responses are elicited, activated, and retained in response to mucosal inflammation from persistent MCMV infection of the submaxillary gland.
Affiliations:Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia 23501, USA.
Reference Type:Literature
PubMed ID:12525604
Journal:J Virol
Journal Volume:77
Article Pages:1703-17
Journal ISSN:1098-5514
Article Chemical List:Antigens, CD3;Interleukin-10;Interferon-gamma
Article MeSH List:3T3 Cells; Animals; Antigen-Presenting Cells(physiology); Antigens, CD3(analysis); CD4-Positive T-Lymphocytes(immunology); Interferon-gamma(biosynthesis); Interleukin-10(biosynthesis); Lymph Nodes(immunology); Male; Mice; Mice, Inbred BALB C; Muromegalovirus(immunology); Submandibular Gland(virology); T-Lymphocytes, Cytotoxic(immunology)
Curation Last Updated:2015-07-30 20:47:28