Epitopes described in "Vaccination with a MHC class II peptide attenuates cellular and humoral responses against tAChR and suppresses clinical EAMG."

Article Authors:Minako Oshima; Philip Deitiker; Tetsuo Ashizawa; M Zouhair Atassi
Article Title:Vaccination with a MHC class II peptide attenuates cellular and humoral responses against tAChR and suppresses clinical EAMG.
Reference Detail
Reference ID:1016896
Abstract:An animal model of myasthenia gravis (MG), termed experimental autoimmune MG (EAMG), can be induced in C57BL/6 (B6, H-2b) mice by immunization with Torpedo californica acetylcholine receptor (tAChR). We have investigated the effect of vaccination with MHC class II peptide I-A beta(b)62-76 on clinical EAMG and on T cell and antibody (Ab) responses against tAChR. B6 mice were vaccinated with the peptide (25 microg/mouse) four times prior to two injections with tAChR. The incidence of clinical EAMG in vaccinated mice was 14% (3 out of 22 mice) compared to 48% (17 out of 35 mice) in control non-vaccinated or PBS-immunized mice. The T cells of the vaccinated group showed lower proliferative responses to tAChR and to T-cell epitope-containing tAChR alpha-chain peptides than the T cells of controls. In addition, the Ab responses in the vaccinated group was also lower against tAChR and some of the B-cell epitope-containing tAChR alpha-chain peptides.
Affiliations:Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, USA. moshima@bcm.tmc.edu
Reference Type:Literature
PubMed ID:12389643
Journal Volume:35
Article Pages:183-90
Journal ISSN:0891-6934
Article Chemical List:Histocompatibility Antigens Class II;Peptide Fragments;Receptors, Cholinergic
Article MeSH List:Amino Acid Sequence; Animals; Antibody Formation; Histocompatibility Antigens Class II(immunology); Lymphocyte Activation; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Myasthenia Gravis, Autoimmune, Experimental(prevention & control); Peptide Fragments(immunology); Receptors, Cholinergic(immunology); T-Lymphocytes(immunology); Torpedo; Vaccination
Curation Last Updated:2016-02-22 20:03:17