Epitopes described in "Identification of sequential IgE-binding epitopes on bovine alpha(s2)-casein in cow's milk allergic patients."

Article Authors:Paula J Busse; Kirsi-Marjut Järvinen; Leticia Vila; Kirsten Beyer; Hugh A Sampson
Article Title:Identification of sequential IgE-binding epitopes on bovine alpha(s2)-casein in cow's milk allergic patients.
Reference Detail
Reference ID:1005471
Abstract:BACKGROUND: Caseins are the major allergens responsible for cow's milk allergy (CMA). We have previously identified the IgE-binding epitopes of the major cow's milk (CM) proteins except for alpha(s2)-casein. METHODS: Overlapping decapeptides representing the entire length of alpha(s2)-casein were synthesized on a cellulose-derivatized membrane. Sera from 13 CM-allergic children, 4-15 years of age, with a median level of CM-specific IgE >100 kU/l (range 33.7 to > 100 kU/l) were used to identify IgE-binding epitopes. RESULTS: Four major and six minor sequential IgE-binding regions were identified on alpha(s2)-casein. The first major region is located in the middle of the protein at amino acids (AA) 83-100, and the other three major regions are located in the carboxy terminal portion of the protein at AA 143-158, 157-172 and 165-188. The minor IgE-binding regions were identified at AA 31-44, 43-56, 93-106, 105-114, 117-128, and 191-200. CONCLUSION: We identified 10 sequential IgE-binding regions on alpha(s2)-casein and performed the first crucial step in the development of immunotherapeutic interventions for CMA.
Affiliations:Department of Pediatrics, Division of Allergy and Immunology, Jaffe Institute for Food Allergy, The Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029-6574, USA.
Reference Type:Literature
PubMed ID:12373003
Journal:Int Arch Allergy Immunol
Journal Volume:129
Article Pages:93-6
Journal ISSN:1423-0097
Article Chemical List:Caseins;Epitopes, B-Lymphocyte;Immunoglobulin E
Article MeSH List:Adolescent; Amino Acid Sequence; Animals; Binding Sites, Antibody; Caseins(chemistry; immunology); Cattle; Child; Child, Preschool; Epitopes, B-Lymphocyte; Female; Humans; Immunoglobulin E(metabolism); Male; Milk Hypersensitivity(etiology); Molecular Sequence Data
Curation Last Updated:2014-10-03 20:41:04