Epitopes described in "Identification of Epstein-Barr virus-specific CD8+ T lymphocytes in the circulation of pediatric transplant recipients."

Article Authors:Daniel A Falco; Ronald R Nepomuceno; Sheri M Krams; Peter P Lee; Mark M Davis; Oscar Salvatierra; Steven R Alexander; Carlos O Esquivel; Kenneth L Cox; Lorry R Frankel; Olivia M Martinez
Article Title:Identification of Epstein-Barr virus-specific CD8+ T lymphocytes in the circulation of pediatric transplant recipients.
Reference Detail
Reference ID:1004679
Abstract:BACKGROUND: Pediatric transplant recipients are at increased risk for Epstein Barr virus (EBV)-related B cell lymphomas. In healthy individuals, the expansion of EBV-infected B cells is controlled by CD8+ cytotoxic T cells. However, immunosuppressive therapy may compromise antiviral immunity. We identified and determined the frequency of EBV-specific T cells in the peripheral blood of pediatric transplant recipients. METHODS: HLA-B*0801 and HLA-A*0201 tetramers folded with immunodominant EBV peptides were used to detect EBV-specific CD8+ T cells by flow cytometry in peripheral blood mononuclear cells from 24 pediatric liver and kidney transplant recipients. The expression of CD38 and CD45RO on EBV-specific, tetramer-binding cells was also examined in a subset of patients by immunofluorescent staining and flow cytometry. RESULTS: Tetramer-binding CD8+ T cells were identified in 21 of 24 transplant recipients. EBV-specific CD8+ T cells were detected as early as 4 weeks after transplant in EBV seronegative patients receiving an organ from an EBV seropositive donor. The frequencies (expressed as a percentage of the CD8+ T cells) of the tetramer-binding cells were HLA-B8-RAKFKQLL (BZLF1 lytic antigen peptide) tetramer, range=0.96 to 3.94%; HLA-B8-FLRGRAYGL (EBNA3A latent antigen peptide) tetramer, range=0.03 to 0.59%; and HLA-A2-GLCTLVAML (BMLF1 lytic antigen peptide) tetramer, range=0.06 to 0.76%. The majority of tetramer reactive cells displayed an activated/memory phenotype. CONCLUSIONS: Pediatric transplant recipients receiving immunosuppression can generate EBV-specific CD8+ T cells. Phenotypic and functional analysis of tetramer cells may prove useful in defining and monitoring EBV infection in the posttransplant patient.
Affiliations:Department of Pediatrics, Stanford University School of Medicine, CA 94305, USA.
Reference Type:Literature
PubMed ID:12352909
Journal Volume:74
Article Pages:501-10
Journal ISSN:0041-1337
Article Chemical List:Antigens, CD;Antigens, Differentiation;HLA-A2 Antigen;HLA-B8 Antigen;Membrane Glycoproteins;ADP-ribosyl Cyclase;Antigens, CD38;CD38 protein, human;NAD+ Nucleosidase
Article MeSH List:ADP-ribosyl Cyclase; Adolescent; Antigens, CD; Antigens, CD38; Antigens, Differentiation(analysis); CD8-Positive T-Lymphocytes(immunology); Child; Child, Preschool; HLA-A2 Antigen(chemistry); HLA-B8 Antigen(chemistry); Herpesvirus 4, Human(immunology); Humans; Immunophenotyping; Infant; Kidney Transplantation(adverse effects); Liver Transplantation(adverse effects); Lymphoma, B-Cell(diagnosis); Membrane Glycoproteins; NAD+ Nucleosidase(analysis)
Curation Last Updated:2014-10-03 20:34:43