Epitopes described in "Celiac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues."

Article Authors:Helene Arentz-Hansen; Stephen N McAdam; Øyvind Molberg; Burkhard Fleckenstein; Knut E A Lundin; Thomas J D Jørgensen; Günther Jung; Peter Roepstorff; Ludvig M Sollid
Article Title:Celiac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues.
Reference Detail
Reference ID:1005477
Abstract:BACKGROUND & AIMS: Celiac disease is a gluten-induced enteropathy that shows a strong association with HLA-DQ2 and -DQ8. Gluten-specific T cells, invariably restricted by DQ2 or DQ8, can be isolated from celiac lesions. Such gut-derived T cells have a preference for recognition of gluten that has been specifically deamidated by tissue transglutaminase. Only a few gliadin T-cell epitopes have been identified by earlier work. The aim of this study was to perform a systematic characterization of DQ2-restricted T-cell epitopes in alpha- and gamma-gliadins. METHODS: Epitopes were identified by mass spectrometry analysis of peptide fragments of recombinant gliadins and by use of synthetic peptides. RESULTS: We identified several new gamma-gliadin epitopes and an additional alpha-gliadin epitope. Interestingly, these and the previously identified epitopes are not randomly scattered across the gliadins but cluster in regions of the proteins with high content of proline residues. CONCLUSIONS: Several DQ2-restricted T-cell epitopes exist in gliadin that are located in regions rich in proline. This likely reflects epitope selection at the levels of digestive and antigen-presenting cell processing, transglutaminase-mediated deamidation, and/or peptide binding to DQ2.
Reference Type:Literature
PubMed ID:12198706
Journal Volume:123
Article Pages:803-9
Journal ISSN:0016-5085
Article Chemical List:Epitopes;HLA-DQ Antigens;HLA-DQ2 antigen;Recombinant Proteins;Gliadin;Proline
Article MeSH List:Amino Acid Sequence(genetics); Celiac Disease(immunology); Epitopes(immunology); Gliadin(genetics; immunology); HLA-DQ Antigens(immunology); Humans; Molecular Sequence Data; Proline; Recombinant Proteins; T-Lymphocytes(immunology)
Curation Last Updated:2014-10-03 20:41:07