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| Article Authors: | Helene Arentz-Hansen; Stephen N McAdam; Øyvind Molberg; Burkhard Fleckenstein; Knut E A Lundin; Thomas J D Jørgensen; Günther Jung; Peter Roepstorff; Ludvig M Sollid |
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| Article Title: | Celiac lesion T cells recognize epitopes that cluster in regions of gliadins rich in proline residues. |
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| Reference ID: | 1005477 |
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| Abstract: | BACKGROUND & AIMS: Celiac disease is a gluten-induced enteropathy that shows a strong association with HLA-DQ2 and -DQ8. Gluten-specific T cells, invariably restricted by DQ2 or DQ8, can be isolated from celiac lesions. Such gut-derived T cells have a preference for recognition of gluten that has been specifically deamidated by tissue transglutaminase. Only a few gliadin T-cell epitopes have been identified by earlier work. The aim of this study was to perform a systematic characterization of DQ2-restricted T-cell epitopes in alpha- and gamma-gliadins. METHODS: Epitopes were identified by mass spectrometry analysis of peptide fragments of recombinant gliadins and by use of synthetic peptides. RESULTS: We identified several new gamma-gliadin epitopes and an additional alpha-gliadin epitope. Interestingly, these and the previously identified epitopes are not randomly scattered across the gliadins but cluster in regions of the proteins with high content of proline residues. CONCLUSIONS: Several DQ2-restricted T-cell epitopes exist in gliadin that are located in regions rich in proline. This likely reflects epitope selection at the levels of digestive and antigen-presenting cell processing, transglutaminase-mediated deamidation, and/or peptide binding to DQ2. |
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| Affiliations: | Institute of Immunology and Department of Medicine, Rikshopitalet, University of Oslo, Oslo, Norway. |
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| Date: | 2002 |
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| Reference Type: | Literature |
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| PubMed ID: | 12198706 |
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| Journal: | Gastroenterology |
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| Journal Volume: | 123 |
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| Article Pages: | 803-9 |
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| Journal ISSN: | 0016-5085 |
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| Article Chemical List: | Epitopes;HLA-DQ Antigens;HLA-DQ2 antigen, human;Recombinant Proteins;Proline;Gliadin |
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| Article MeSH List: | Amino Acid Sequence(genetics ); Celiac Disease(immunology ); Epitopes(immunology ); Gliadin(genetics; immunology ); HLA-DQ Antigens(immunology ); Humans; Molecular Sequence Data; Proline; Recombinant Proteins; T-Lymphocytes(immunology ) |
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| Curation Last Updated: | 2011-10-28 20:39:51 |
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