Epitopes described in "Phenotypic and functional heterogeneity of EBV epitope-specific CD8+ T cells."

Article Authors:Michelle D Catalina; John L Sullivan; Robin M Brody; Katherine Luzuriaga
Article Title:Phenotypic and functional heterogeneity of EBV epitope-specific CD8+ T cells.
Reference Detail
Reference ID:1004718
Abstract:High frequencies of EBV-specific CD8(+) T cells have been detected during acute EBV infection, yet persistent infection inevitably results. To address this issue, we characterized the phenotype and function of epitope-specific CD8(+) T cell populations from presentation with acute through latent infection. Considerable phenotypic and functional heterogeneity within, as well as between, two different epitope-specific populations was observed over time following acute infection. B7 EBV-encoded nuclear Ag (EBNA)-3A-specific CD8(+) T cells expressed only CD45RO from acute through latent EBV infection. A2 BMLF-1-specific CD8(+) T cells expressed CD45RO during acute infection and either CD45RA or CD45RO during latent EBV infection. This difference in CD45 isoform expression between the two epitope-specific populations did not translate into differences in perforin content, the ability to produce IFN-gamma, or the ability to proliferate in response to Ag in vitro. In individuals with latent EBV infection, the frequencies of A2 BMLF-1- or B7 EBNA-3A-specific CD8(+) T cells that expressed CD45RA, CD45RO, CD62 ligand, CCR7, and perforin were stable over time. However, the expression of CD62 ligand and CCR7 was significantly higher among EBNA-3A-specific CD8(+) T cells than among BMLF-1-specific CD8(+) T cells. Further work is necessary to understand how phenotypic and functional differences between EBV epitope-specific CD8(+) T cells are related to the biology of the virus and to the equilibrium between the virus and the host during persistent infection.
Affiliations:Department of Pediatrics and Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Reference Type:Literature
PubMed ID:11937579
Journal:J Immunol
Journal Volume:168
Article Pages:4184-91
Journal ISSN:0022-1767
Article Chemical List:Antigens, CD80;Epitopes, T-Lymphocyte;Epstein-Barr Virus Nuclear Antigens;HLA-A2 Antigen;Isoenzymes;Membrane Glycoproteins;Oligopeptides;Pore Forming Cytotoxic Proteins;Receptors, Lymphocyte Homing;glycyl-leucyl-cysteinyl-threonyl-leucyl-valyl-alanyl-methionyl-leucine;Perforin;Interferon-gamma;Antigens, CD45
Article MeSH List:Adolescent; Adult; Antigens, CD45(biosynthesis); Antigens, CD80(metabolism); CD8-Positive T-Lymphocytes(enzymology; immunology; metabolism; virology); Cell Line, Transformed; Cell Membrane(enzymology; immunology; metabolism); Epitopes, T-Lymphocyte(biosynthesis; immunology); Epstein-Barr Virus Nuclear Antigens(metabolism); HLA-A2 Antigen(metabolism); Herpesvirus 4, Human(immunology); Humans; Immunologic Memory; Immunophenotyping; Interferon-gamma(biosynthesis); Isoenzymes(biosynthesis); Lymphocyte Activation; Membrane Glycoproteins(biosynthesis; metabolism); Oligopeptides(metabolism); Perforin; Pore Forming Cytotoxic Proteins; Protein Binding(immunology); Receptors, Lymphocyte Homing(biosynthesis); T-Lymphocyte Subsets(enzymology; immunology; metabolism; virology)
Curation Last Updated:2015-09-25 18:04:43