Epitopes described in "Resolution of chronic hepatitis B and anti-HBs seroconversion in humans by adoptive transfer of immunity to hepatitis B core antigen."

Article Authors:George K K Lau; Deepak Suri; Raymond Liang; Eirini I Rigopoulou; Mark G Thomas; Ivana Mullerova; Amin Nanji; Siu-Tsan Yuen; Roger Williams; Nikolai V Naoumov
Article Title:Resolution of chronic hepatitis B and anti-HBs seroconversion in humans by adoptive transfer of immunity to hepatitis B core antigen.
Reference Detail
Reference ID:1003474
Abstract:BACKGROUND & AIMS: Impaired T-cell reactivity is believed to be the dominant cause of chronic hepatitis B virus (HBV) infection. We characterized HBV-specific T-cell responses in chronic hepatitis B surface antigen carriers who received bone marrow from HLA-identical donors with natural immunity to HBV and seroconverted to antibody to hepatitis B surface antigen. METHODS: T-cell reactivity to HBV antigens and peptides was assessed in a proliferation assay, the frequency of HBV core- and surface-specific T cells was quantified directly by ELISPOT assays, and T-cell subsets were analyzed by flow cytometry. RESULTS: CD4+ T-cell reactivity to HBV core was common in bone marrow donors and the corresponding recipients after hepatitis B surface antigen clearance, whereas none reacted to surface, pre-S1, or pre-S2 antigens. Furthermore, CD4+ T cells from donor/recipient pairs recognized similar epitopes on hepatitis B core antigen; using polymerase chain reaction for the Y chromosome, the recipients' CD4+ T lymphocytes were confirmed to be of donor origin. The frequency of core-specific CD4+ and CD8+ T cells was several-fold higher than those specific for surface antigen. CONCLUSIONS: This study provides the first evidence in humans that transfer of hepatitis B core antigen-reactive T cells is associated with resolution of chronic HBV infection. Therapeutic immunization with HBV core gene or protein deserves further investigation in patients with chronic hepatitis B.
Affiliations:Institute of Hepatology, University College London, London, England.
Reference Type:Literature
PubMed ID:11874993
Journal Volume:122
Article Pages:614-24
Journal ISSN:0016-5085
Article Chemical List:Hepatitis B Antibodies;Hepatitis B Core Antigens
Article MeSH List:Adolescent; Adult; Bone Marrow Transplantation; CD4-Positive T-Lymphocytes(cytology; immunology; virology ); CD8-Positive T-Lymphocytes(cytology; immunology; virology ); Cell Division(immunology ); Female; Flow Cytometry; Hepatitis B Antibodies(blood ); Hepatitis B Core Antigens(immunology ); Hepatitis B, Chronic(immunology; therapy ); Humans; Immunotherapy, Adoptive; Lymphocyte Activation(immunology ); Male; Middle Aged
Curation Last Updated:2015-01-27 19:03:40