Epitopes described in "A monoclonal antibody that recognizes a potential coeliac-toxic repetitive pentapeptide epitope in gliadins."

Article Authors:A A Osman; H H Uhlig; I Valdes; M Amin; E Méndez; T Mothes
Article Title:A monoclonal antibody that recognizes a potential coeliac-toxic repetitive pentapeptide epitope in gliadins.
Reference Detail
Reference ID:1005517
Abstract:OBJECTIVES: Antibodies that detect coeliac-toxic prolamins from wheat, barley and rye are important tools for controlling the diet of coeliac disease patients. Recently, a monoclonal antibody R5 that recognizes wheat gliadin, barley hordein and rye secalin equally was described. In this study, the epitope recognized by R5 was investigated. METHODS: Both a phage-displayed heptapeptide library and overlapping peptides spanning the sequence of alpha- and gamma-type gliadins (pepscan) were screened for binding of R5. RESULTS: Both techniques yielded comparable pentapeptide consensus sequences (phage display QXPW/FP; pepscan QQPFP). According to recent observations, this peptide stretch may be of key importance in the pathogenicity of coeliac disease. This sequence occurs repetitively in prolamins (in gamma- and omega-type prolamins more frequently than in alpha-type prolamins) together with several homologous peptide stretches, which are recognized less strongly. CONCLUSIONS: R5 seems to be a good candidate for the specific detection of putative coeliac disease-active sequences in prolamins and thus represents a valuable tool for the quality control of gluten-free food.
Affiliations:Department of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Leipzig, Germany.
Reference Type:Literature
PubMed ID:11711775
Journal:Eur J Gastroenterol Hepatol
Journal Volume:13
Article Pages:1189-93
Journal ISSN:0954-691X
Article Chemical List:Antibodies, Monoclonal;Epitopes;Glutens;Gliadin
Article MeSH List:Amino Acid Sequence; Antibodies, Monoclonal(metabolism); Celiac Disease(genetics; immunology); Enzyme-Linked Immunosorbent Assay; Epitopes(genetics; immunology); Food Analysis; Gliadin(genetics; immunology); Glutens(genetics); Humans; Molecular Sequence Data
Curation Last Updated:2014-10-03 20:41:18