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| Article Authors: | A A Osman; H H Uhlig; I Valdes; M Amin; E Méndez; T Mothes |
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| Article Title: | A monoclonal antibody that recognizes a potential coeliac-toxic repetitive pentapeptide epitope in gliadins. |
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| Reference ID: | 1005517 |
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| Abstract: | OBJECTIVES: Antibodies that detect coeliac-toxic prolamins from wheat, barley and rye are important tools for controlling the diet of coeliac disease patients. Recently, a monoclonal antibody R5 that recognizes wheat gliadin, barley hordein and rye secalin equally was described. In this study, the epitope recognized by R5 was investigated. METHODS: Both a phage-displayed heptapeptide library and overlapping peptides spanning the sequence of alpha- and gamma-type gliadins (pepscan) were screened for binding of R5. RESULTS: Both techniques yielded comparable pentapeptide consensus sequences (phage display QXPW/FP; pepscan QQPFP). According to recent observations, this peptide stretch may be of key importance in the pathogenicity of coeliac disease. This sequence occurs repetitively in prolamins (in gamma- and omega-type prolamins more frequently than in alpha-type prolamins) together with several homologous peptide stretches, which are recognized less strongly. CONCLUSIONS: R5 seems to be a good candidate for the specific detection of putative coeliac disease-active sequences in prolamins and thus represents a valuable tool for the quality control of gluten-free food. |
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| Affiliations: | Department of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital, Leipzig, Germany. |
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| Date: | 2001 |
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| Reference Type: | Literature |
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| PubMed ID: | 11711775 |
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| Journal: | Eur J Gastroenterol Hepatol |
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| Journal Volume: | 13 |
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| Article Pages: | 1189-93 |
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| Journal ISSN: | 0954-691X |
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| Article Chemical List: | Antibodies, Monoclonal;Epitopes;Glutens;Gliadin |
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| Article MeSH List: | Amino Acid Sequence; Antibodies, Monoclonal(metabolism); Celiac Disease(genetics; immunology); Enzyme-Linked Immunosorbent Assay; Epitopes(genetics; immunology); Food Analysis; Gliadin(genetics; immunology); Glutens(genetics); Humans; Molecular Sequence Data |
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| Curation Last Updated: | 2012-12-17 20:01:41 |
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