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| Article Authors: | J T Voeten; G F Rimmelzwaan; N J Nieuwkoop; R A Fouchier; A D Osterhaus |
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| Article Title: | Antigen processing for MHC class I restricted presentation of exogenous influenza A virus nucleoprotein by B-lymphoblastoid cells. |
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| Reference ID: | 1153 |
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| Abstract: | In general, exogenous proteins are processed by antigen-presenting cells in the endosomes for major histocompatibility complex (MHC) class II presentation to CD4+ T cells, while proteins synthesized endogenously are processed in the cytoplasm for MHC class I presentation to CD8+ T cells. However, it is recognized that exogenous proteins can be processed for MHC class I presentation also, and evidence in favour of alternatives to the conventional MHC class I processing and presentation pathway is accumulating. Here, we show that exogenous recombinant influenza A virus nucleoprotein (rNP) is processed for MHC class I presentation to CD8+ cytotoxic T lymphocytes (CTL) by EBV-transformed, B-lymphoblastoid cell lines (B-LCL). Processing of rNP for HLA-B27-associated presentation seemed to follow the conventional MHC class I pathway predominantly, as presentation was diminished in the presence of lactacystin and brefeldin A, but was less sensitive to chloroquine and NH4Cl. HLA-B27-associated presentation was also observed using cells lacking a functional transporter associated with antigen processing, suggesting that alternative pathways may be exploited for processing of rNP. |
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| Affiliations: | Institute of Virology and WHO National Influenza Centre, Erasmus Medical Centre Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands. |
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| Date: | 2001 |
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| Reference Type: | Literature |
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| PubMed ID: | 11531950 |
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| Journal: | Clin Exp Immunol |
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| Journal Volume: | 125 |
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| Article Pages: | 423-31 |
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| Journal ISSN: | 1365-2249 |
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| Article Chemical List: | Enzyme Inhibitors;Histocompatibility Antigens Class I;NP protein, Influenza A virus;Nucleoproteins;Peptide Fragments;RNA-Binding Proteins;Viral Core Proteins;lactacystin;Brefeldin A;Chloroquine;Acetylcysteine |
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| Article MeSH List: | Acetylcysteine(analogs & derivatives; pharmacology); Antigen Presentation(drug effects; immunology); B-Lymphocytes(immunology); Brefeldin A(pharmacology); Chloroquine(pharmacology); Endosomes(drug effects); Enzyme Inhibitors(pharmacology); Hematopoietic Stem Cells(immunology); Histocompatibility Antigens Class I(immunology); Humans; Influenza A virus(immunology); Major Histocompatibility Complex; Nucleoproteins(immunology); Peptide Fragments(immunology); RNA-Binding Proteins; Viral Core Proteins(immunology) |
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| Curation Last Updated: | 2013-05-28 20:05:48 |
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