Epitopes described in "Antigen processing for MHC class I restricted presentation of exogenous influenza A virus nucleoprotein by B-lymphoblastoid cells."

Article Authors:J T Voeten; G F Rimmelzwaan; N J Nieuwkoop; R A Fouchier; A D Osterhaus
Article Title:Antigen processing for MHC class I restricted presentation of exogenous influenza A virus nucleoprotein by B-lymphoblastoid cells.
Reference Detail
Reference ID:1153
Abstract:In general, exogenous proteins are processed by antigen-presenting cells in the endosomes for major histocompatibility complex (MHC) class II presentation to CD4+ T cells, while proteins synthesized endogenously are processed in the cytoplasm for MHC class I presentation to CD8+ T cells. However, it is recognized that exogenous proteins can be processed for MHC class I presentation also, and evidence in favour of alternatives to the conventional MHC class I processing and presentation pathway is accumulating. Here, we show that exogenous recombinant influenza A virus nucleoprotein (rNP) is processed for MHC class I presentation to CD8+ cytotoxic T lymphocytes (CTL) by EBV-transformed, B-lymphoblastoid cell lines (B-LCL). Processing of rNP for HLA-B27-associated presentation seemed to follow the conventional MHC class I pathway predominantly, as presentation was diminished in the presence of lactacystin and brefeldin A, but was less sensitive to chloroquine and NH4Cl. HLA-B27-associated presentation was also observed using cells lacking a functional transporter associated with antigen processing, suggesting that alternative pathways may be exploited for processing of rNP.
Affiliations:Institute of Virology and WHO National Influenza Centre, Erasmus Medical Centre Rotterdam, Dr Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.
Reference Type:Literature
PubMed ID:11531950
Journal:Clin Exp Immunol
Journal Volume:125
Article Pages:423-31
Journal ISSN:0009-9104
Article Chemical List:Enzyme Inhibitors;Histocompatibility Antigens Class I;NP protein, Influenza A virus;Nucleoproteins;Peptide Fragments;RNA-Binding Proteins;Viral Core Proteins;lactacystin;Brefeldin A;Chloroquine;Acetylcysteine
Article MeSH List:Acetylcysteine(analogs & derivatives; pharmacology); Antigen Presentation(drug effects; immunology); B-Lymphocytes(immunology); Brefeldin A(pharmacology); Chloroquine(pharmacology); Endosomes(drug effects); Enzyme Inhibitors(pharmacology); Hematopoietic Stem Cells(immunology); Histocompatibility Antigens Class I(immunology); Humans; Influenza A virus(immunology); Major Histocompatibility Complex; Nucleoproteins(immunology); Peptide Fragments(immunology); RNA-Binding Proteins; Viral Core Proteins(immunology)
Curation Last Updated:2015-06-05 00:14:38