Epitopes described in "Swift development of protective effector functions in naive CD8(+) T cells against malaria liver stages."

Article Authors:G Sano; J C Hafalla; A Morrot; R Abe; J J Lafaille; F Zavala
Article Title:Swift development of protective effector functions in naive CD8(+) T cells against malaria liver stages.
Reference Detail
Reference ID:1025834
Abstract:We generated T cell receptor transgenic mice specific for the liver stages of the rodent malaria parasite Plasmodium yoelii and studied the early events in the development of in vivo effector functions in antigen-specific CD8(+) T cells. Differently to activated/memory cells, naive CD8(+) T cells are not capable of exerting antiparasitic activity unless previously primed by parasite immunization. While naive cells need to differentiate before achieving effector status, the time required for this process is very short. Indeed, interferon (IFN)-gamma and perforin mRNA are detectable 24 h after immunization and IFN-gamma secretion and cytotoxic activity are detected ex vivo 24 and 48 h after immunization, respectively. In contrast, the proliferation of CD8(+) T cells begins after 24 h and an increase in the total number of antigen-specific cells is detected only after 48 h. Remarkably, a strong CD8(+) T cell-mediated inhibition of parasite development is observed in mice challenged with viable parasites only 24 h after immunization with attenuated parasites. These results indicate that differentiation of naive CD8(+) T cells does not begin only after extensive cell division, rather this process precedes or occurs simultaneously with proliferation.
Affiliations:Department of Medical and Molecular Parasitology, New York University School of Medicine, New York, NY 10010, USA.
Reference Type:Literature
PubMed ID:11457892
Journal:J Exp Med
Journal Volume:194
Article Pages:173-80
Journal ISSN:0022-1007
Article Chemical List:Antigens, Protozoan;DNA Primers;Epitopes;Membrane Glycoproteins;Pore Forming Cytotoxic Proteins;RNA, Messenger;Receptors, Antigen, T-Cell, alpha-beta;Perforin;Interferon-gamma
Article MeSH List:Amino Acid Sequence; Animals; Antigens, Protozoan(genetics); Base Sequence; CD8-Positive T-Lymphocytes(cytology; immunology); Cell Differentiation; Cell Division; DNA Primers(genetics); Epitopes(genetics); Immunization; Interferon-gamma(biosynthesis; genetics); Liver(parasitology); Lymphocyte Activation; Malaria(immunology; parasitology); Membrane Glycoproteins(genetics); Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Perforin; Plasmodium yoelii(genetics; growth & development; immunology; pathogenicity); Pore Forming Cytotoxic Proteins; RNA, Messenger(genetics; metabolism); Receptors, Antigen, T-Cell, alpha-beta(genetics)
Curation Last Updated:2015-07-30 20:47:30