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| Article Authors: | E J Novak; A W Liu; J A Gebe; B A Falk; G T Nepom; D M Koelle; W W Kwok |
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| Article Title: | Tetramer-guided epitope mapping: rapid identification and characterization of immunodominant CD4+ T cell epitopes from complex antigens. |
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| Reference ID: | 1443 |
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| Abstract: | T cell responses to Ags involve recognition of selected peptide epitopes contained within the antigenic protein. In this report, we describe a new approach for direct identification of CD4+ T cell epitopes of complex Ags that uses human class II tetramers to identify reactive cells. With a panel of 60 overlapping peptides covering the entire sequence of the VP16 protein, a major Ag for HSV-2, we generated a panel of class II MHC tetramers loaded with peptide pools that were used to stain peripheral lymphocytes of an HSV-2 infected individual. With this approach, we identified four new DRA1*0101/DRB1*0401- and two DRA1*0101/DRB1*0404-restricted, VP16-specific epitopes. By using tetramers to sort individual cells, we easily obtained a large number of clones specific to these epitopes. Although DRA1*0101/DRB1*0401 and DRA1*0101/DRB1*0404 are structurally very similar, nonoverlapping VP16 epitopes were identified, illustrating high selectivity of individual allele polymorphisms within common MHC variants. This rapid approach to detecting CD4+ T cell epitopes from complex Ags can be applied to any known Ag that gives a T cell response. |
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| Affiliations: | Virginia Mason Research Center, Benaroya Research Institute, Seattle, WA 98101, USA. |
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| Date: | 2001 |
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| Reference Type: | Literature |
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| PubMed ID: | 11359821 |
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| Journal: | J Immunol |
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| Journal Volume: | 166 |
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| Article Pages: | 6665-70 |
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| Journal ISSN: | 0022-1767 |
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| Article Chemical List: | Epitopes, T-Lymphocyte;HLA-DR Antigens;HLA-DRA;Herpes Simplex Virus Protein Vmw65;Immunodominant Epitopes;Macromolecular Substances;Peptide Fragments;HLA-DRB1 antigen |
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| Article MeSH List: | CD4-Positive T-Lymphocytes(chemistry; metabolism; virology); Cell Line, Transformed; Clone Cells(chemistry; immunology); Epitope Mapping(methods); Epitopes, T-Lymphocyte(analysis; chemistry; metabolism); HLA-DR Antigens(analysis; biosynthesis; metabolism); Herpes Simplex Virus Protein Vmw65(analysis; biosynthesis; immunology; metabolism); Herpesvirus 2, Human(immunology); Humans; Immunodominant Epitopes(analysis; chemistry; metabolism); Lymphocyte Activation; Macromolecular Substances; Peptide Fragments(biosynthesis; immunology; metabolism); Protein Binding(immunology) |
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| Curation Last Updated: | 2013-05-28 20:07:25 |
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