Epitopes described in "Tetramer-guided epitope mapping: rapid identification and characterization of immunodominant CD4+ T cell epitopes from complex antigens."

Article Authors:E J Novak; A W Liu; J A Gebe; B A Falk; G T Nepom; D M Koelle; W W Kwok
Article Title:Tetramer-guided epitope mapping: rapid identification and characterization of immunodominant CD4+ T cell epitopes from complex antigens.
Reference Detail
Reference ID:1443
Abstract:T cell responses to Ags involve recognition of selected peptide epitopes contained within the antigenic protein. In this report, we describe a new approach for direct identification of CD4+ T cell epitopes of complex Ags that uses human class II tetramers to identify reactive cells. With a panel of 60 overlapping peptides covering the entire sequence of the VP16 protein, a major Ag for HSV-2, we generated a panel of class II MHC tetramers loaded with peptide pools that were used to stain peripheral lymphocytes of an HSV-2 infected individual. With this approach, we identified four new DRA1*0101/DRB1*0401- and two DRA1*0101/DRB1*0404-restricted, VP16-specific epitopes. By using tetramers to sort individual cells, we easily obtained a large number of clones specific to these epitopes. Although DRA1*0101/DRB1*0401 and DRA1*0101/DRB1*0404 are structurally very similar, nonoverlapping VP16 epitopes were identified, illustrating high selectivity of individual allele polymorphisms within common MHC variants. This rapid approach to detecting CD4+ T cell epitopes from complex Ags can be applied to any known Ag that gives a T cell response.
Affiliations:Virginia Mason Research Center, Benaroya Research Institute, Seattle, WA 98101, USA.
Reference Type:Literature
PubMed ID:11359821
Journal:J Immunol
Journal Volume:166
Article Pages:6665-70
Journal ISSN:0022-1767
Article Chemical List:Epitopes, T-Lymphocyte;HLA-DR Antigens;HLA-DR alpha-Chains;HLA-DRB1 Chains;HLA-DRB1*04:01 antigen;Herpes Simplex Virus Protein Vmw65;Immunodominant Epitopes;Macromolecular Substances;Peptide Fragments
Article MeSH List:CD4-Positive T-Lymphocytes(chemistry; metabolism; virology); Cell Line, Transformed; Clone Cells(chemistry; immunology); Epitope Mapping(methods); Epitopes, T-Lymphocyte(analysis; chemistry; metabolism); HLA-DR Antigens(analysis; biosynthesis; metabolism); HLA-DR alpha-Chains; HLA-DRB1 Chains; Herpes Simplex Virus Protein Vmw65(analysis; biosynthesis; immunology; metabolism); Herpesvirus 2, Human(immunology); Humans; Immunodominant Epitopes(analysis; chemistry; metabolism); Lymphocyte Activation; Macromolecular Substances; Peptide Fragments(biosynthesis; immunology; metabolism); Protein Binding(immunology)
Curation Last Updated:2014-10-03 19:40:07