Epitopes described in "Secretion of anti-citrulline-containing peptide antibody by B lymphocytes in rheumatoid arthritis."

Article Authors:C C Reparon-Schuijt; W J van Esch; C van Kooten; G A Schellekens; B A de Jong; W J van Venrooij; F C Breedveld; C L Verweij
Article Title:Secretion of anti-citrulline-containing peptide antibody by B lymphocytes in rheumatoid arthritis.
Reference Detail
Reference ID:1015101
Abstract:OBJECTIVE: To understand the regulation of anti-citrulline-containing peptide antibody (anti-CCP) production in rheumatoid arthritis (RA), production of anti-CCP by B cells derived from peripheral blood (PB), bone marrow (BM), and synovial fluid (SF) was examined. METHODS: Purified PB and SF B cells were isolated by negative selection and then cultured in the absence or presence of L-CD40 ligand cells and interleukin-10 or anti-CD3-activated T cells. Total IgM and IgM-anti-CCP were detected after 14 days of culture by enzyme-linked immunosorbent assay. Enzyme-linked immunospot assays were performed to analyze the frequency of cells that spontaneously produced IgM-anti-CCP in BM and SF B cells. RESULTS: IgM-anti-CCP autoantibodies were induced in PB B cells from healthy controls and RA patients following coculture with activated T cells or application of the CD40 activation system, whereas no production could be detected when PB B cells were cultured in the absence of a stimulus. SF and BM B cells from anti-CCP-seropositive RA patients, but not anti-CCP-seronegative patients, actively produced IgM-anti-CCP without stimulation. The frequency of spontaneous production of IgM-anti-CCP among the IgM-secreting cells ranged from 2.2% to 25%. CONCLUSION: These results indicate the presence of B cell precursors for anti-CCP autoantibodies that are able to produce antibodies upon stimulation in the PB B cell repertoire of healthy controls and patients with RA. In contrast, B cells that actively secreted anti-CCP were specifically present in the BM and SF compartment of anti-CCP-seropositive RA patients. The local presence of anti-CCP-secreting cells in the inflamed joints provides evidence for an antigen-driven maturation of CCP-specific B cells at the site of inflammation in RA.
Reference Type:Literature
PubMed ID:11212174
Journal:Arthritis Rheum
Journal Volume:44
Article Pages:41-7
Journal ISSN:0004-3591
Article Chemical List:Autoantibodies;Immunoglobulin M;Citrulline
Article MeSH List:Antibody Formation; Arthritis, Rheumatoid(blood); Autoantibodies(metabolism); B-Lymphocytes(metabolism); Citrulline(immunology); Humans; Immunoglobulin M(blood); Synovial Fluid(cytology)
Curation Last Updated:2015-12-28 20:02:18