Epitopes described in "Protective and nonprotective epitopes from amino termini of M proteins from Australian aboriginal isolates and reference strains of group A streptococci."

Article Authors:E R Brandt; T Teh; W A Relf; R I Hobb; M F Good
Article Title:Protective and nonprotective epitopes from amino termini of M proteins from Australian aboriginal isolates and reference strains of group A streptococci.
Reference Detail
Reference ID:1002002
Abstract:The M protein is the primary vaccine candidate to prevent group A streptococcal (GAS) infection and the subsequent development of rheumatic fever (RF). However, the large number of serotypes have made it difficult to design a vaccine against all strains. We have taken an approach of identifying amino-terminal M protein epitopes from GAS isolates that are highly prevalent in GAS-endemic populations within the Northern Territory (NT) of Australia. Australian Aboriginals in the NT experience the highest incidence of RF worldwide. To develop a vaccine for this population, 39 peptides were synthesized, representing the amino-terminal region of the M protein from endemic GAS. Mice immunized with these peptides covalently linked to tetanus toxoid and emulsified in complete Freund's adjuvant raised high-titer antibodies. Over half of these sera reduced bacterial colony counts by >80% against the homologous isolate of GAS. Seven of the peptide antisera also cross-reacted with at least three other heterologous peptides by enzyme-linked immunosorbent assay. Antiserum to one peptide, BSA10(1-28), could recognize six other peptides, and five of these peptides could inhibit opsonization mediated by BSA10(1-28) antiserum. Cross-opsonization studies showed that six of these sera could opsonize at least one heterologous isolate of GAS. These data reveal vaccine candidates specific to a GAS-endemic area and show the potential of some to cross-opsonize multiple isolates of GAS. This information will be critical when considering which epitopes may be useful in a multiepitope vaccine to prevent GAS infection.
Reference Type:Literature
PubMed ID:11083769
Journal:Infect Immun
Journal Volume:68
Article Pages:6587-94
Journal ISSN:1098-5522
Article Chemical List:Antigens, Bacterial;Bacterial Outer Membrane Proteins;Bacterial Proteins;Carrier Proteins;Epitopes;Immune Sera;Peptide Fragments;Streptococcal Vaccines;streptococcal M protein
Article MeSH List:Amino Acid Sequence; Animals; Antigens, Bacterial; Bacterial Outer Membrane Proteins; Bacterial Proteins(chemistry; immunology); Blood Bactericidal Activity; Carrier Proteins(chemistry; immunology); Cross Reactions; Enzyme-Linked Immunosorbent Assay; Epitopes; Immune Sera(immunology); Mice; Molecular Sequence Data; Peptide Fragments(immunology); Streptococcal Vaccines(immunology); Streptococcus pyogenes(immunology)
Curation Last Updated:2014-10-03 20:14:51