Epitopes described in "UTY gene codes for an HLA-B60-restricted human male-specific minor histocompatibility antigen involved in stem cell graft rejection: characterization of the critical polymorphic amino acid residues for T-cell recognition."

Reference
Article Authors:M H Vogt; E Goulmy; F M Kloosterboer; E Blokland; R A de Paus; R Willemze; J H Falkenburg
Article Title:UTY gene codes for an HLA-B60-restricted human male-specific minor histocompatibility antigen involved in stem cell graft rejection: characterization of the critical polymorphic amino acid residues for T-cell recognition.
Reference Detail
Reference ID:315591
Abstract:Rejection of a graft after human leukocyte antigen (HLA)-identical stem cell transplantation (SCT) can be caused by recipient's immunocompetent T lymphocytes recognizing minor histocompatibility antigens on donor stem cells. During rejection of a male stem cell graft by a female recipient, 2 male (H-Y)-specific cytotoxic T lymphocyte (CTL) clones were isolated from peripheral blood. One CTL clone recognized an HLA-A2-restricted H-Y antigen, encoded by the SMCY gene. Another CTL clone recognized an HLA-B60-restricted H-Y antigen. In this study UTY was identified as the gene coding for the HLA-B60-restricted H-Y antigen. The UTY-derived H-Y antigen was characterized as a 10-amino acid residue peptide, RESEEESVSL. Although the epitope differed by 3 amino acids from its X-homologue, UTX, only 2 polymorphisms were essential for recognition by the CTL clone HLA-B60 HY. These results illustrate that CTLs against several H-Y antigens derived from different proteins can contribute simultaneously to graft rejection after HLA-identical, sex-mismatched SCT. Moreover, RESEEESVSL-specific T cells could be isolated from a female HLA-B60+ patient with myelodysplastic syndrome who has been treated with multiple blood transfusions, but not from control healthy HLA-B60+ female donors. This may indicate that RESEEESVSL-reactive T cells are more common in sensitized patients.
Affiliations:Departments of Hematology and Immunohematology and Bloodbank, Leiden University Medical Center, Leiden, The Netherlands. m.h.j.vogt@lumc.nl
Date:2000
Reference Type:Literature
PubMed ID:11049993
Journal:Blood
Journal Volume:96
Article Pages:3126-32
Journal ISSN:1528-0020
Article Chemical List:H-Y Antigen;HLA-B Antigens;HLA-B60 antigen;Minor Histocompatibility Antigens;Nuclear Proteins;Oligodeoxyribonucleotides;Proteins;UTY protein, human
Article MeSH List:Base Sequence; Cloning, Molecular; Female; Graft Rejection(genetics; immunology); H-Y Antigen(genetics); HLA-B Antigens(genetics); HeLa Cells; Hematopoietic Stem Cell Transplantation; Humans; Male; Minor Histocompatibility Antigens(genetics); Molecular Sequence Data; Nuclear Proteins; Oligodeoxyribonucleotides(genetics); Polymorphism, Genetic; Proteins(chemistry; genetics); T-Lymphocytes(immunology); T-Lymphocytes, Cytotoxic(immunology); Transfection; Y Chromosome
Article Comments:Data originally imported from the HLA Ligand Database (http://hlaligand.ouhsc.edu/)
Curation Last Updated:2013-06-21 20:03:37