Epitopes described in "Intraocular T cells of patients with herpes simplex virus (HSV)-induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14."

Article Authors:G M Verjans; M E Dings; J McLauchlan; A van Der Kooi; P Hoogerhout; H F Brugghe; H A Timmermans; G S Baarsma; A D Osterhaus
Article Title:Intraocular T cells of patients with herpes simplex virus (HSV)-induced acute retinal necrosis recognize HSV tegument proteins VP11/12 and VP13/14.
Reference Detail
Reference ID:1004324
Abstract:It has previously been shown that T cells specific for the triggering virus infiltrate the eye of patients with herpes simplex virus type 1 (HSV-1)-induced acute retinal necrosis (ARN). The T cells were mainly directed against 0.67-0.73 HSV-1 map region encoded antigens. The fine specificities of genetically different T cell clones (TCC), obtained from affected eyes of 3 patients with HSV-induced ARN and reactive toward this genomic region of HSV-1, were analyzed with recombinant HSV viruses and synthetic peptides. For 1 patient, the HSV-1 UL46 gene encoded tegument protein VP11/12 was identified as the target antigen. Two separate CD4(+) T cell epitopes were defined in VP11/12. TCC from the other 2 patients recognized the HSV-1 UL47 gene encoded tegument protein VP13/14. Two separate CD4(+) VP13/14 T cell epitopes were identified in these patients. Analysis of the data indicates that HSV-1 VP11/12 and VP13/14 are major target antigens for T cells obtained from vitreous fluid samples of the HSV-induced ARN patients studied.
Affiliations:Institute of Virology, Erasmus University Rotterdam, 3000 DR Rotterdam, The Netherlands. Verjans@viro.fgg.eur.nl
Reference Type:Literature
PubMed ID:10950790
Journal:J Infect Dis
Journal Volume:182
Article Pages:923-7
Journal ISSN:0022-1899
Article Chemical List:Antigens, Viral;Epitopes;Receptors, Antigen, T-Cell, alpha-beta;UL46 protein, Human herpesvirus 1;VP13-14 protein, herpes simplex virus type 1;Viral Fusion Proteins;Viral Proteins
Article MeSH List:Antigens, Viral; Cells, Cultured; Cytotoxicity, Immunologic; Epitopes; Eye(immunology; pathology ); Herpesvirus 1, Human(genetics; immunology ); Humans; Lymphocyte Activation; Phenotype; Receptors, Antigen, T-Cell, alpha-beta(immunology ); Retinal Necrosis Syndrome, Acute(immunology; pathology ); T-Lymphocytes(immunology ); Viral Fusion Proteins(immunology ); Viral Proteins
Curation Last Updated:2015-01-27 19:11:01