Epitopes described in "HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells."

Reference
Article Authors:W W Kwok; A W Liu; E J Novak; J A Gebe; R A Ettinger; G T Nepom; S N Reymond; D M Koelle
Article Title:HLA-DQ tetramers identify epitope-specific T cells in peripheral blood of herpes simplex virus type 2-infected individuals: direct detection of immunodominant antigen-responsive cells.
Reference Detail
Reference ID:1000781
Abstract:Ag-specific CD4+ T cells are present in peripheral blood in low frequency, where they undergo recruitment and expansion during immune responses and in the pathogenesis of numerous autoimmune diseases. MHC tetramers, which constitute a labeled MHC-peptide ligand suitable for binding to the Ag-specific receptor on T cells, provide a novel approach for the detection and characterization of such rare cells. In this study, we utilized this technology to identify HLA DQ-restricted Ag-specific T cells in the peripheral blood of human subjects and to identify immunodominant epitopes associated with viral infection. Peptides representing potential epitope regions of the VP16 protein from HSV-2 were loaded onto recombinant DQ0602 molecules to generate a panel of Ag-specific DQ0602 tetramers. VP16 Ag-specific DQ-restricted T cells were identified and expanded from the peripheral blood of HSV-2-infected individuals, representing two predominant epitope specificities. Although the VP16 369-380 peptide has a lower binding affinity for DQ0602 molecules than the VP16 33-52 peptide, T cells that recognized the VP16 369-380 peptide occurred at a much higher frequency than those that were specific for the VP16 33-52 peptide.
Affiliations:Virginia Mason Research Center, Seattle, WA 98101, USA. bkwok@vmresearch.org
Date:2000
Reference Type:Literature
PubMed ID:10754321
Journal:J Immunol
Journal Volume:164
Article Pages:4244-9
Journal ISSN:0022-1767
Article Chemical List:Antigens, Viral;Epitopes, T-Lymphocyte;HLA-DQ Antigens;Herpes Simplex Virus Protein Vmw65;Immunodominant Epitopes;Peptide Fragments
Article MeSH List:Amino Acid Sequence; Antigens, Viral(immunology); Clone Cells; Epitopes, T-Lymphocyte(blood); HLA-DQ Antigens(blood; genetics; immunology); Herpes Genitalis(blood; immunology); Herpes Simplex Virus Protein Vmw65(immunology; metabolism); Herpesvirus 2, Human(immunology); Humans; Immunodominant Epitopes(blood); Influenza A virus(immunology); Lymphocyte Count; Molecular Sequence Data; Peptide Fragments(immunology; metabolism); Protein Binding(immunology); Stem Cells(immunology; metabolism); T-Lymphocytes(immunology; metabolism)
Curation Last Updated:2014-07-16 20:25:44