Epitopes described in "The human UTY gene encodes a novel HLA-B8-restricted H-Y antigen."

Article Authors:E H Warren; M A Gavin; E Simpson; P Chandler; D C Page; C Disteche; K A Stankey; P D Greenberg; S R Riddell
Article Title:The human UTY gene encodes a novel HLA-B8-restricted H-Y antigen.
Reference Detail
Reference ID:1020145
Abstract:The mammalian Y chromosome encodes male-specific minor histocompatibility (H-Y) Ags that are recognized by female T cells in an MHC-restricted manner. Two human H-Y epitopes presented by HLA-A2 and HLA-B7, respectively, have been identified previously and both are derived from the SMCY gene. We previously isolated CD8+ CTL clones that recognized a male-specific minor histocompatibility Ag presented by HLA-B8. In contrast to the SMCY-encoded H-Y epitopes, the B8/H-Y Ag was not presented by fibroblasts from male donors, suggesting that it was encoded by a novel gene. We now report that the HLA-B8-restricted H-Y epitope is defined by the octameric peptide LPHNHTDL corresponding to aa residues 566-573 of the human UTY protein. Transcription of the UTY gene is detected in a wide range of human tissues, but presentation of the UTY-derived H-Y epitope to CTL by cultured human cells shows significant cell-type specificity. Identification of this CTL-defined H-Y epitope should facilitate analysis of its contribution to graft/host interactions following sex-mismatched organ and bone marrow transplantation.
Affiliations:Program in Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. ehwarren@u.washington.edu
Reference Type:Literature
PubMed ID:10679124
Journal:J Immunol
Journal Volume:164
Article Pages:2807-14
Journal ISSN:0022-1767
Article Chemical List:Epitopes;H-Y Antigen;HLA-B8 Antigen;Nuclear Proteins;Oligopeptides;Proteins;UTY protein, human
Article MeSH List:Amino Acid Sequence; Animals; COS Cells; Cell Line, Transformed; Cells, Cultured; Chromosome Mapping; Epitopes(biosynthesis; genetics); Female; Gene Expression Regulation(immunology); H-Y Antigen(biosynthesis; genetics); HLA-B8 Antigen(biosynthesis; genetics); Humans; Male; Molecular Sequence Data; Nuclear Proteins; Oligopeptides(genetics; immunology; isolation & purification); Organ Specificity(genetics; immunology); Protein Biosynthesis; Proteins(genetics; immunology); Y Chromosome(immunology)
Curation Last Updated:2014-10-03 22:10:12