Epitopes described in "Cutting edge: the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique."

Reference
Article Authors:R A Pierce; E D Field; J M den Haan; J A Caldwell; F M White; J A Marto; W Wang; L M Frost; E Blokland; C Reinhardus; J Shabanowitz; D F Hunt; E Goulmy; V H Engelhard
Article Title:Cutting edge: the HLA-A*0101-restricted HY minor histocompatibility antigen originates from DFFRY and contains a cysteinylated cysteine residue as identified by a novel mass spectrometric technique.
Reference Detail
Reference ID:315335
Abstract:In this report, we describe the use of novel mass spectrometry instrumentation to identify a male-specific minor histocompatibility Ag restricted by HLA-A*0101 (A1-HY). This Ag has the sequence IVDC*LTEMY, where C* represents a cysteine disulfide bonded to a second cysteine residue. The core peptide sequence is found in the protein product of DFFRY, a Y chromosome gene not previously identified as the source of an HY Ag. The male-specific form of the peptide differs from its X chromosomal counterpart by the substitution of serine for the C* residue. Both peptides are expressed on the cell surface at 30 or fewer copies per cell. However, A1-HY-specific CTL recognize the DFFRY-derived peptide at a 1500-fold lower dose than the female homologue. Thus, these studies have identified a new source of HY epitopes and provide additional information about the influence of posttranslational modifications of class I-associated peptides on T cell recognition.
Date:1999
Reference Type:Literature
PubMed ID:10586024
Journal:J Immunol
Journal Volume:163
Article Pages:6360-4
Journal ISSN:0022-1767
Article Chemical List:Antigens, Surface;Disulfides;Epitopes;H-Y Antigen;HLA-A Antigens;Methionine;Cysteine
Article MeSH List:Antigens, Surface(isolation & purification; metabolism); Cells, Cultured; Cysteine(metabolism); Disulfides(metabolism); Epitopes(isolation & purification); Female; H-Y Antigen(isolation & purification; metabolism); HLA-A Antigens(immunology); Humans; Male; Mass Spectrometry(methods); Methionine(metabolism); X Chromosome(genetics); Y Chromosome(genetics)
Article Comments:Data originally imported from the Database of Functional Molecular Immunology, FIMM (http://sdmc.lit.org.sg:8080/fimm/)
Curation Last Updated:2014-04-01 18:08:27