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| Article Authors: | M Christodoulides; E Rattue; J E Heckels |
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| Article Title: | Effect of adjuvant composition on immune response to a multiple antigen peptide (MAP) containing a protective epitope from Neisseria meningitidis class 1 porin. |
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| Reference ID: | 1008917 |
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| Abstract: | A variety of adjuvants with the potential for use with experimental human vaccines were used for immunisation of mice, in an attempt to augment the humoral immune response to a multiple antigen peptide (MAP) containing a protective epitope from the sero-subtype specific class 1 porin protein of Neisseria meningitidis, in tandem with a Th-cell epitope. Surface plasmon resonance showed that combinations of the immunomodulators pluronic block co-polymer, muramyl dipeptide and monophosphoryl lipid A (MPL), increased the magnitude and avidity of the immune response in comparison with both Al(OH)3 and Freund-type adjuvants. In addition, the incorporation of MPL was essential for the induction of a broad distribution of antibody isotypes. The antibodies induced recognised the native protein in meningococcal outer membranes in a subtype-specific manner. The formulations containing these multiple immunomodulators which have already been used in human phase I/II trials with experimental vaccines, are candidates for inclusion in future human vaccines based on synthetic peptides containing defined, protective epitopes. |
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| Affiliations: | Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, UK. mc4@soton.ac.uk |
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| Date: | 1999 |
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| Reference Type: | Literature |
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| PubMed ID: | 10501243 |
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| Journal: | Vaccine |
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| Journal Volume: | 18 |
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| Article Pages: | 131-9 |
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| Journal ISSN: | 0264-410X |
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| Article Chemical List: | Adjuvants, Immunologic;Antibodies, Bacterial;Bacterial Outer Membrane Proteins;Bacterial Vaccines;Epitopes;Immune Sera;Porins |
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| Article MeSH List: | Adjuvants, Immunologic(pharmacology ); Animals; Antibodies, Bacterial(blood; immunology ); Bacterial Outer Membrane Proteins(immunology ); Bacterial Vaccines(immunology ); Epitopes; Immune Sera(immunology ); Mice; Mice, Inbred BALB C; Neisseria meningitidis(immunology ); Porins(immunology ) |
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| Curation Last Updated: | 2009-11-09 19:30:51 |
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